MITOSIS IN NORMAL AND REGENERATING PLANARIANS
MITOSIS IN NORMAL AND REGENERATING PLANARIANS
复制标题
DOI：
10.1002/jez.1401680204
复制
发表时间：
1968-01-01
影响因子：
--
通讯作者：
ROSENVOLD, R
中科院分区：
文献类型：
--
作者：
BEST, JB;HAND, S;ROSENVOLD, R
用两种方法测定涡虫有丝分裂率。首先，通过在秋水仙碱中不同时间后显示中期有丝分裂图形的细胞核比例的制表。第二，测量一群涡虫的总DNA随时间的变化。第一种方法的有丝分裂率为1.4 × 10−4次/细胞小时。该速率在斩首后的前24小时内保持不变，但在54小时内增加到2.7 × 10−4个分裂/细胞小时。胚芽有丝分裂率不大于老部分间充质组织有丝分裂率。总DNA随时间的变化，估计自发裂变涡虫的平均细胞分裂速率为5.8 × 10−4次/细胞小时，考虑到秋水仙碱法有低估真实速率的倾向，这一结果是令人满意的。讨论了这些结果对再生机制的意义。
The rate of mitosis in planarians was measured by two methods. First, by tabulating the proportion of nuclei displaying metaphase mitotic figures after varying lengths of time in colchicine. Second, by measuring the total DNA in a colony of planarians as a function of time. The first method gave a rate of mitosis by non‐regeneratingDugesia dorotocephalaof 1.4 × 10−4divisions/cell hour. This rate remains constant for the first 24 hours following decapitation but increases to 2.7 × 10−4divisions/cell hour by 54 hours. The rate of mitosis in the blastema was no larger than that of the mesenchymal tissues of the older portions. Measurements of the total DNA versus time gave an estimate of the average rate of cell division for spontaneously fissioning planarians of 5.8 × 10−4divisions/cell hour which, in view of the tendency of the colchicine method to underestimate the true rate, is in satisfactory agreement. The implications of these results for mechanisms of regeneration are discussed.