Flavin Monooxygenase-Generated N-Hydroxypipecolic Acid Is a Critical Element of Plant Systemic Immunity
Flavin Monooxygenase-Generated N-Hydroxypipecolic Acid Is a Critical Element of Plant Systemic Immunity
复制标题
DOI：
10.1016/j.cell.2018.02.049
复制
发表时间：
2018-04-05
期刊：
影响因子：
64.5
通讯作者：
Zeier, Juergen
中科院分区：
文献类型：
--
作者：
Hartmann, Michael;Zeier, Tatyana;Zeier, Juergen
在先前的微生物接种后，植物可以诱导对病原体感染的广谱免疫，这种现象被称为系统性获得性抗性（SAR）。拟南芥SAR的建立受赖氨酸分解代谢物黑果酸（Pip）和黄素依赖单氧酶1 （FMO1）的调控。在这里，我们发现升高的Pip足以诱导叶片依赖于FMO1的转录重编程，这让人想起SAR。在植物和体外分析中，FMO1作为一种胡椒酸n -羟化酶，催化Pip向n -羟基果酸（NHP）的生化转化。微生物攻击后，植物体内会系统性地积累nhp。当外源施用时，它可以克服nhp缺乏的fmo1在获得性抗性中的缺陷，并作为植物对细菌和卵菌感染免疫的有效诱导剂。我们的工作已经确定了植物中病原体诱导的l -赖氨酸分解代谢途径，该途径产生n-羟基化氨基酸NHP，作为对病原体感染的系统性获得性抗性的关键调节因子。
Following a previous microbial inoculation, plants can induce broad-spectrum immunity to pathogen infection, a phenomenon known as systemic acquired resistance (SAR). SAR establishment in Arabidopsis thaliana is regulated by the Lys catabolite pipecolic acid (Pip) and flavin-dependent-monooxygenase1 (FMO1). Here, we show that elevated Pip is sufficient to induce an FMO1-dependent transcriptional reprogramming of leaves that is reminiscent of SAR. In planta and in vitro analyses demonstrate that FMO1 functions as a pipecolate N-hydroxylase, catalyzing the biochemical conversion of Pip to N-hydroxypipecolic acid (NHP). NHP-systemically accumulates in plants after microbial attack. When exogenously applied, it overrides the defect of NHP-deficient fmo1 in acquired resistance and acts as a potent inducer of plant immunity to bacterial and oomycete infection. Our work has identified a pathogen-inducible L-Lys catabolic pathway in plants that generates the N-hydroxylated amino acid NHP as a critical regulator of systemic acquired resistance to pathogen infection.