Gephyronic acid, a novel inhibitor of eukaryotic protein synthesis from Archangium gephyra (myxobacteria). Production, isolation, physico-chemical and biological properties, and mechanism of action.
Gephyronic acid, a novel inhibitor of eukaryotic protein synthesis from Archangium gephyra (myxobacteria). Production, isolation, physico-chemical and biological properties, and mechanism of action.
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Gephyronic Acid 是一种来自Archangium gephyra（粘细菌）的新型真核蛋白质合成抑制剂。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
H. Reichenbach
中科院分区：
文献类型：
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作者：
F. Sasse;H. Steinmetz;G. Höfle;H. Reichenbach
从粘杆菌 Archangium gephyra 菌株 Ar 3895 的培养液中分离出一种新的抗生素化合物 gephyronic Acid。在对数生长期和稳定生长期，产量高达 3 毫克/升。该化合物是一种脂肪酸，易于形成半缩醛。两种形式均抑制酵母和霉菌的生长（MIC 1-25 微克/ml），并对哺乳动物细胞培养物具有细胞抑制作用（IC50 10-60 ng/ml）。 Gephyronic Acid 是一种真核蛋白质合成的特异性抑制剂，在体外翻译测定中显示 IC50 为 1-2 x 10(-7) mol/L。
A new antibiotic compound, gephyronic acid was isolated from the culture broth of the myxobacterium, Archangium gephyra strain Ar 3895. Up to 3 mg/liter was produced during the logarithmic and stationary growth phase. The compound is an aliphatic acid, which tends to form a hemiacetal. Both forms inhibited growth of yeasts and molds (MIC 1-25 micrograms/ml) and had a cytostatic effect on mammalian cell cultures (IC50 10-60 ng/ml). Gephyronic acid is a specific inhibitor of eukaryotic protein synthesis showing an IC50 of 1-2 x 10(-7) mol/liter in an in vitro translation assay.