Gamma interferon induces detectable serological and functional expression of DR and DP but not DQ antigens on cultured amniotic fluid cells.
Gamma interferon induces detectable serological and functional expression of DR and DP but not DQ antigens on cultured amniotic fluid cells.
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γ干扰素在培养的羊水细胞上诱导可检测到的 DR 和 DP 的血清学和功能表达，但不诱导 DQ 抗原。
DOI：
10.1111/j.1399-0039.1988.tb02079.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Pollack,MS
中科院分区：
文献类型：
--
作者：
Maurer,DH;Callaway,C;Sorkin,S;Pollack,MS
培养的羊水细胞通常含有成纤维细胞和上皮样细胞的混合物，用于HLA相关疾病产前诊断和亲子鉴定的HLA分型研究。两种类型的细胞都缺乏HLA II类抗原的组成性表达，但这些可以通过γ干扰素预处理诱导。血清学和功能研究表明，可检测水平的DR和DP，但不能检测到DQ抗原。然而，DR和DP不能在绒毛膜绒毛细胞上诱导。
Cultured amniotic fluid cells, which are used for HLA typing studies for the prenatal diagnosis of HLA linked diseases and for prenatal determination of paternity, usually contain mixtures of fibroblastic and epithelioid cells. Cells of both types lack constitutive expression of HLA class II antigens, but these can be induced by pretreatment with gamma interferon. Both serological and functional studies indicate that detectable levels of DR and DP but not DQ antigens can be thereby induced. DR and DP cannot, however, be induced on chorionic villus cells.