Seroprevalence of enterovirus 71 and no evidence of crossprotection of enterovirus 71 antibody against the other enteroviruses in kindergarten children in Taipei city
Seroprevalence of enterovirus 71 and no evidence of crossprotection of enterovirus 71 antibody against the other enteroviruses in kindergarten children in Taipei city
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DOI：
10.1016/j.jmii.2011.09.025
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发表时间：
2012-04-01
影响因子：
7.4
通讯作者：
Chang, Luan-Yin
中科院分区：
文献类型：
--
作者：
Huang, Wen-Chan;Huang, Li-Min;Chang, Luan-Yin
背景/目的：肠道病毒71型（EV 71）感染可引起严重的神经和心肺并发症，尤其是在学龄前儿童中。本研究旨在探讨肠道病毒71型的血清阳性率、血清转换率及肠道病毒71型抗体对其他肠道病毒的交叉保护作用。方法：以台北市一所公立幼儿园2006、2007两个学年共228名幼儿为研究对象，检测其肠道病毒71型中和抗体。当参与者患有疱疹性咽峡炎、手足口病（HFMD）、发热性疾病或呼吸道症状时，对咽拭子进行采样和处理，用于病毒培养和肠道病毒实时逆转录酶聚合酶链反应（RT-PCR）。结果：2006年和2007年的EV 71血清阳性率分别为20%（32/163）和6%（4/65）。2006年，EV 71血清阳性率随年龄增长而增加（p < 0.01），但性别之间没有差异（p = 0.14）。2006年至2007年期间未观察到血清转化。疱疹性咽峡炎发生在64%的EV 71血清阳性儿童和48%的EV 71抗体阴性儿童（p = 0.12）。经病毒研究证实，53%（19/36）的EV 71血清阳性儿童和53%（102/192）的EV 71血清阴性儿童发生非EV 71肠道病毒感染（p = 0.89）。结论：2006年9月至2008年6月期间，台北市肠病毒71型并未频繁传播。EV 71中和抗体的存在与非71型肠道病毒感染的发生率较低无关。Copyright（c）2011，Taiwan Society of Microbiology.出版社：Elsevier Taiwan LLC All rights reserved.
Background/Purpose: Enterovirus 71 (EV71) infection may cause severe neurological and cardiopulmonary complications, especially in preschool children. This study is to investigate the seroprevalence and seroconversion of EV71, and the crossprotection of EV71 antibody against other enteroviruses among kindergarteners.Methods: Overall 228 children in a public kindergarten were enrolled during two academic years, 2006 and 2007, in Taipei, Taiwan and we measured their EV71 neutralizing antibody. When the participants had herpangina; hand, foot and mouth disease (HFMD); febrile illness or respiratory symptoms, throat swabs were sampled and processed for viral culture and enterovirus real-time reverse transcriptase polymerase chain reaction (RT-PCR). Questionnaires, completed by the participants' guardians, surveyed the history of allergy and annual incidence of symptoms related to enterovirus infection.Results: Seropositive rates of EV71 were 20% (32/163) in 2006 and 6% (4/65) in 2007. The rate of EV71 seropositivity increased with age (p < 0.01) in 2006 but it did not differ between genders (p = 0.14). No seroconversion was observed from 2006 to 2007. Herpangina occurred in 64% of children with EV71 seropositivity and 48% of those without EV71 antibodies (p = 0.12). Non-71 enterovirus infection, confirmed by viral study, occurred in 53% (19/36) of the EV71-seropositive children and in 53% (102/192) of EV71-seronegative children (p = 0.89). No participants had EV71 infection during the study period.Conclusion: EV71 did not frequently circulate in Taipei City from September 2006 to June 2008. Presence of EV71 neutralizing antibody was not associated with lower incidence of enterovirus infection caused by non-71 serotypes. Copyright (c) 2011, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.