Mitochondrial metabolic states regulate nitric oxide and hydrogen peroxide diffusion to the cytosol
Mitochondrial metabolic states regulate nitric oxide and hydrogen peroxide diffusion to the cytosol
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DOI：
10.1016/j.bbabio.2006.02.010
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发表时间：
2006-05-01
影响因子：
4.3
通讯作者：
Bustamante, Juanita
中科院分区：
文献类型：
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作者：
Boveris, Alberto;Valdez, Laura B.;Bustamante, Juanita
从大鼠心、肝、肾和脑(呼吸控制4.0-6.5)分离的线粒体释放NO和H_2O_2的速率取决于线粒体的代谢状态：状态4释放的NO约为状态3的1.7-2.0倍，H_2O_2的释放是状态3的4-16倍。用Rh-123荧光测定，大鼠肝线粒体NO的释放在55-180 mV范围内与膜电位呈指数依赖关系。线粒体过氧化氢的产生也有类似的行为[S.S.Korshunov，VP。Skulachev，A.A.Starkov，高质子势激活了线粒体中产生活性氧物种的机制。费布斯·莱特。416(1997)15-18。]。通过流式细胞仪和DiOC6和X-1荧光检测，大脑皮层线粒体从状态4到状态3的转变与NO释放量(50%)和膜电位(24-53%)的下降有关。线粒体扩散提供细胞内NO的比例在心脏为61%，在肝脏为47%，在肾脏为30%，在脑为18%。这些数据支持这样的推测，即NO和过氧化氢报告了高线粒体能量电荷到细胞质中。膜电位调节线粒体一氧化氮合酶活性，使线粒体一氧化氮合酶成为可调控酶，进而调节线粒体O2摄取和过氧化氢的产生。(C)2006爱思唯尔B.V.保留所有权利。
Mitochondria isolated from rat heart, liver, kidney and brain (respiratory control 4.0-6.5) release NO and H2O2 At rates that depend on the mitochondrial metabolic state: releases are higher in state 4, about 1.7-2.0 tirnes for NO and 4-16 times for H2O2, than in state 3. NO release in rat liver mitochondria showed an exponential dependence on membrane potential in the range 55 to 180 mV, as determined by Rh-123 fluorescence. A similar behavior was reported for mitochondrial H2O2 production by [S.S. Korshunov, VP. Skulachev, A.A. Starkov, High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria. FEBS Lett. 416 (1997) 15_18.]. Transition from state 4 to state 3 of brain cortex mitochondria was associated to a decrease in NO release (50%) and in membrane potential (24-53%), this latter determined by flow cytometry and DiOC6 and X-1 fluorescence. The fraction of cytosolic NO provided by diffusion from mitochondria was 61% in heart, 47% in liver, 30% in kidney, and 18% in brain. The data supports the speculation that NO and H2O2 report a high mitochondrial energy charge to the cytosol. Regulation of mtNOS activity by membrane potential makes mtNOS a regulable enzyme that in turn regulates mitochondrial 02 uptake and H2O2 production. (c) 2006 Elsevier B.V. All rights reserved.