Immunodepressive effect of 3-methylcholanthrene. Antibody formation at the cellular level and reaction against weak antigenic homografts.
Immunodepressive effect of 3-methylcholanthrene. Antibody formation at the cellular level and reaction against weak antigenic homografts.
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3-甲基胆蒽的免疫抑制作用。
DOI：
10.1093/jnci/35.5.885
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发表时间：
1965
期刊：
影响因子：
--
通讯作者：
Jan Stjernswärd
中科院分区：
文献类型：
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作者：
Jan Stjernswärd
在用单细胞水平测量的绵羊红细胞免疫后，给予小鼠3-甲基二蒽（MCA）可减少产生抗体的脾细胞数量。免疫抑制是快速而持久的。即使在 MCA 暴露 2 天后，当给予测试抗原时，形成空斑的脾细胞 (PFC) 的数量也减少了 50% 以上。单次暴露于 MCA 后，PFC 在一段时间内保持抑制，这段时间相当于 MCA 诱发的肉瘤出现之前的潜伏期。在给予 MCA 后 1 至 20 周的不同时间间隔，对小鼠组中的这种 PFC 抑制进行了研究。脾脏重量和脾细胞总数减少，并且当以脾细胞总数的分数表示时，PFC 有特定的减少。将细胞免疫反应的抑制与针对异源测试抗原羊红细胞的体液溶血和血凝抗体反应进行比较。通过植皮在单特异性弱组织相容性屏障 (H-1) 上进行测试，MCA 暴露后移植物排斥反应的损害在肿瘤出现时首次得到证实。在肿瘤潜伏期的早期阶段，皮肤移植物的存活率没有受到影响。这些发现与 Prehn 和 Main 的早期建议相关讨论，即 MCA 抑制免疫反应，从而允许抗原性肿瘤细胞生长。
3-Methyldiolanthrene (MCA) administered to mice reduced the number of antibody-producing spleen cells after immunization with sheep erythrocytes measured at a single cellular level. The immuno-depression was rapid and long. Even 2 days after MCA exposure, when test antigen was given, the number of plaque-forming spleen cells (PFC) decreased more than 50 percent. After a single exposure to MCA, the PFC remained depressed for a period corresponding to the latency period prior to the appearance of the MCA-induced sarcomas. This depression of PFC was studied in groups of mice at different intervals between 1 and 20 weeks after MCA administration. Spleen weight and total spleen cell number were reduced, and there was a specific reduction of PFC when expressed as a fraction of the total number of spleen cells. The depression of the cellular immune response was compared with the humoral hemolysing and hemagglutinating antibody response toward a heterologous test antigen, sheep erythrocytes. Impairment of the graft rejection after MCA exposure, tested across a monospecific weak histocompatibility barrier (H-1) by skin grafting, was first demonstrable at the time of tumor appearance. Skingraft survival was not affected during the earlier part of the tumor latency period. The findings are discussed in relation to an earlier suggestion of Prehn and Main that MCA inhibits immunological responses and thereby allows the outgrowth of antigenic neoplastic cells.