Evidence for selection on coloration in a Panamanian poison frog: a coalescent-based approach
Evidence for selection on coloration in a Panamanian poison frog: a coalescent-based approach
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DOI：
10.1111/j.1365-2699.2009.02260.x
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发表时间：
2010-05-01
影响因子：
3.9
通讯作者：
Summers, Kyle
中科院分区：
文献类型：
--
作者：
Brown, Jason L.;Maan, Martine E.;Summers, Kyle
草莓毒蛙，Oophaga pumilio，在巴拿马的博卡斯德尔托罗群岛经历了显着的辐射颜色变形。这一物种在颜色和图案上有着极大的差异，这些差异在地理上与世隔绝了不到10，000年的种群之间。虽然以前的研究表明，参与分歧选择，迄今为止没有定量测试已经检查了这一hypothes.LocationBocas del Toro archipelago，Panama.MethodsWe使用一个组合的群体遗传学，地理和表型分析，以测试分歧选择着色在O。pumilio。收集了来自15个不同种群的88个个体的组织样品。利用这些数据，我们开发了一个基因树使用线粒体DNA（mtDNA）的d-环区。利用我们的mtDNA遗传学参数，我们预测了一个假设的核基因的合并着色。我们收集了光谱反射率和身体大小的测量94个人从四个种群和进行了定量分析的表型divergence.ResultsThe mtDNA的d-环树显示相当多的多系性跨种群。群体树内基因树的合并重建揭示了群体间不完全的基因型排序。表型分歧的定量分析显示完整的谱系排序的颜色，但不是身体大小：人口显示非重叠的变化，身体着色的光谱反射率的措施，而身体大小的变化没有分开的人群。模拟的聚结使用参数值来自我们的实证分析表明，在颜色中看到的人群之间的排序水平不能合理地归因于drift.Main conclusionsThese结果意味着分歧的颜色，但不是身体大小，发生在一个更快的速度比预期的中性过程下。我们的研究提供了第一个定量支持的索赔，强大的多样化选择的基础上的颜色变化的草莓毒蛙。
AimThe strawberry poison frog, Oophaga pumilio, has undergone a remarkable radiation of colour morphs in the Bocas del Toro archipelago in Panama. This species shows extreme variation in colour and pattern between populations that have been geographically isolated for < 10,000 years. While previous research has suggested the involvement of divergent selection, to date no quantitative test has examined this hypothesis.LocationBocas del Toro archipelago, Panama.MethodsWe use a combination of population genetics, phylogeography and phenotypic analyses to test for divergent selection in coloration in O. pumilio. Tissue samples of 88 individuals from 15 distinct populations were collected. Using these data, we developed a gene tree using the mitochondrial DNA (mtDNA) d-loop region. Using parameters derived from our mtDNA phylogeny, we predicted the coalescence of a hypothetical nuclear gene underlying coloration. We collected spectral reflectance and body size measurements on 94 individuals from four of the populations and performed a quantitative analysis of phenotypic divergence.ResultsThe mtDNA d-loop tree revealed considerable polyphyly across populations. Coalescent reconstructions of gene trees within population trees revealed incomplete genotypic sorting among populations. The quantitative analysis of phenotypic divergence revealed complete lineage sorting by colour, but not by body size: populations showed non-overlapping variation in spectral reflectance measures of body coloration, while variation in body size did not separate populations. Simulations of the coalescent using parameter values derived from our empirical analyses demonstrated that the level of sorting among populations seen in colour cannot reasonably be attributed to drift.Main conclusionsThese results imply that divergence in colour, but not body size, is occurring at a faster rate than expected under neutral processes. Our study provides the first quantitative support for the claim that strong diversifying selection underlies colour variation in the strawberry poison frog.