Cloning of transforming growth factor-β1 (TGF-β1) and its type II receptor from zebrafish ovary and role of TGF-β1 in Oocyte maturation
Cloning of transforming growth factor-β1 (TGF-β1) and its type II receptor from zebrafish ovary and role of TGF-β1 in Oocyte maturation
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DOI：
10.1210/en.2002-0126
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发表时间：
2003-05-01
期刊：
影响因子：
4.8
通讯作者：
Peng, C
中科院分区：
文献类型：
--
作者：
Kohli, G;Hu, SQ;Peng, C
TGF-β是参与调节多种细胞活动的多功能因子。在哺乳动物中，已知TGF-β调节生殖，包括卵巢功能。TGF-β在低等脊椎动物（如鱼类）中的作用知之甚少。为了研究TGF-β在鱼类繁殖中的作用，使用基于PCR的策略从斑马鱼卵巢中克隆了编码TGF-β 1和II型TGF-β受体（TbetaRII）的cDNA。斑马鱼TGF-β 1的成熟肽区与其他物种的TGF-β 1具有70-85%的同一性。斑马鱼TbetaRII cDNA序列是第一个从鱼类中报道的，并且它在激酶结构域显示出高水平的保守性。利用RT-PCR技术检测了不同发育阶段卵泡中TGF-β 1、TbetaRII及其下游信号分子Smad 2、3和4的mRNA表达。此外，我们还研究了TGF-β 1对卵母细胞成熟的影响。TGF-β 1以剂量和时间依赖性方式显著抑制促性腺激素和17 α，20 β-二羟孕酮诱导的卵母细胞成熟。这些发现首次证明，TGF-β 1在调节鱼类卵母细胞成熟中起作用，并表明TGF-β/Smad信号通路存在于斑马鱼卵巢中。
TGF-beta is a multifunctional factor involved in regulating a variety of cellular activities. In mammals, TGF-beta is known to regulate reproduction, including ovarian functions. The role of TGF-beta in lower vertebrates, such as fish, is poorly understood. To examine the role of TGF-beta in fish reproduction, cDNAs encoding TGF-beta1 and the type II TGF-beta receptor ( TbetaRII) were cloned from the zebrafish ovary using PCR-based strategies. The mature peptide region of the zebrafish TGF-beta1 shows 70-85% identity with TGF-beta1 from other species. The zebrafish TbetaRII cDNA sequence is the first to be reported from a fish species, and it shows a high level of conservation at the kinase domain. Using RT-PCR, we have detected mRNA expression of TGF-beta1, TbetaRII, as well as its downstream signaling molecules Smad2, 3, and 4 in ovarian follicles at different stages of development. In addition, we have examined the effect of TGF-beta1 on oocyte maturation. TGF-beta1 significantly inhibited both gonadotropin- and 17alpha, 20beta-dihydroxyprogesterone-induced oocyte maturation in a dose- and time-dependent manner. These findings demonstrate, for the first time, that TGF-beta1 plays a role in regulating oocyte maturation in fish and suggest that a TGF-beta/Smad signaling pathway is present in the zebrafish ovary.