PHOSPHOLIPID ESTER-LINKED FATTY-ACID PROFILE CHANGES DURING NUTRIENT DEPRIVATION OF VIBRIO-CHOLERAE - INCREASES IN THE TRANS CIS RATIO AND PROPORTIONS OF CYCLOPROPYL FATTY-ACIDS
PHOSPHOLIPID ESTER-LINKED FATTY-ACID PROFILE CHANGES DURING NUTRIENT DEPRIVATION OF VIBRIO-CHOLERAE - INCREASES IN THE TRANS CIS RATIO AND PROPORTIONS OF CYCLOPROPYL FATTY-ACIDS
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DOI：
10.1128/aem.52.4.794-801.1986
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发表时间：
1986-10-01
影响因子：
4.4
通讯作者：
WHITE, DC
中科院分区：
文献类型：
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作者：
GUCKERT, JB;HOOD, MA;WHITE, DC
比较了饥饿0天、7天和30天的霍乱弧菌培养物的磷脂酯连接脂肪酸。当细胞饥饿时，在脂肪酸谱中观察到统计学显著趋势。顺式单烯酸的量下降（例如，16：1 ω 7 c：0天，39%; 7天，18%; 30天，11%）。相反，饱和脂肪酸，环丙基衍生物的顺式单烯脂肪酸，反式单烯脂肪酸增加饥饿期间。例如，16：1 ω 7 t的量为：0天，1%; 7天，13%; 30天，17%;这将16：1 ω 7的反式/顺式比率从0.02（0天）增加至0.70（7天）至1.56（30天）。这可能是由于膜磷脂的顺式单烯脂肪酸的高周转率和这些异构体代谢酶的可用性。因此，在饥饿引起的磷脂损失期间，顺式单烯脂肪酸将被优先利用。合成反式单烯酸（不易被细菌代谢）或将更具挥发性的顺式单烯酸修饰为环丙基衍生物的能力可能是一种生存机制，有助于在饥饿诱导的脂质利用期间维持功能性（尽管结构改变）膜。此外，反式/顺式脂肪酸比率显著大于大多数细菌培养物和环境样品所报道的比率（< 0.1），可以用作饥饿或应激脂质指数。这样的比例可以帮助确定超微细菌和其他报道的自然水生环境中的休眠细胞的营养状况。这些生物可能是海洋和河口沉积物中反式单烯脂肪酸输入的直接来源，与顺式到反式异构化不同。
The phospholipid ester-linked fatty acids of 0-day-, 7-day-, and 30-day-starved cultures of Vibrio cholerae were compared. Statistically significant trends were noted in the fatty acid profiles as the cells starved. The amount of the cis-monoenoic fatty acids declined (e.g., 16:1.omega.7c: 0 day, 39%; 7 day, 18%; 30 day, 11%). In contrast, the saturated fatty acids, the cyclopropyl derivatives of the cis-monoenoic fatty acids, and trans-monoenoic fatty acids increased during starvation. For instance, the amounts of 16:1.omega.7t were: 0 day, 1%; 7 day, 13%; 30 day, 17%; which increased the trans/cis ratio for 16:1.omega.7 from 0.02 (0 day) to 0.70 (7 day) to 1.56 (30 day). This may be due to the reported high turnover rates of cis-monoenoic fatty acids of membrane phospholipids and the availability of enzymes for the metabolism of these isomers. During starvation-induced phospholipid loss, the cis-monoenoic fatty acids would, therefore, be preferentially utilized. The ability to either synthesize trans-monoenoic acids (which are not easily metabolized by bacteria) or modify the more volatile cis-monoenoic acids to their cyclopropyl derivatives may be a survival mechanism which helps maintain a functional (although structurally altered) membrane during starvation-induced lipid utilization. In addition, a trans/cis fatty acid ratio significantly greater than that reported for most bacterial cultures and environmental samples (< 0.1)may be used as a starvation or stress lipid index. Such a ratio could help determine the nutritional status of ultramicrobacteria and other reported dormant cells in natural aquatic environments. These organisms may represent a direct source of trans-monoenoic fatty acid input into marine and estuarine sediments as distinct from cis-to-trans isomerization.