Induction of KLF4 in response to heat stress
Induction of KLF4 in response to heat stress
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DOI：
10.1379/csc-210.1
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发表时间：
2006-12-01
影响因子：
3.8
通讯作者：
Xiao, Xianzhong
中科院分区：
文献类型：
--
作者：
Liu, Ying;Wang, Jing;Xiao, Xianzhong
Kruppel样因子4（KLF 4）是一种进化上保守的含锌指的转录因子，在细胞生长、增殖、分化和胚胎发生中具有多种调节功能。然而，KLF 4对热应激的反应知之甚少。在这项研究中，蛋白质印迹和逆转录-聚合酶链反应进行，以确定KLF 4表达的变化，响应热应激。结果表明，热应激可使KLF 4 mRNA和蛋白水平呈时间依赖性上调。此外，对热休克转录因子1（Hsf 1）基因敲除小鼠的研究表明，热应激诱导KLF 4是由Hsf 1介导的。这一过程发生迅速，表明KLF 4是一个热应激的即时早期反应基因。接下来，针对KLF 4过表达或缺乏的细胞分析KLF 4在热应激条件下的作用。结果表明，KLF 4过表达增加了C2 C12细胞的死亡率，而KLF 4缺乏则降低了热应激条件下C2 C12细胞的损伤，提示KLF 4可能在热应激诱导的细胞损伤中起重要作用，KLF 4可能是一种即刻早期反应基因，在热应激诱导的细胞损伤中起重要作用。
Kruppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. However, little is known about the response of KLF4 to heat stress. In this study, Western blot and reverse transcriptase-polymerase chain reaction were performed to determine the changes in KLF4 expression in response to heat stress. The results showed that heat stress up-regulated KLF4 messenger RNA and protein levels in a time-dependent manner in vivo and in 4 cell lines. Moreover, a study with heat shock transcription factor 1 (Hsf1) gene knockout mice indicated that the induction of KLF4 in response to heat stress was mediated by Hsf1. This process occurred rapidly, indicating that KLF4 is an immediate early response gene of heat stress. Next, the roles of KLF4 under heat stress conditions were analyzed for cells overexpressing or deficient in KLF4. The results showed overexpression of KLF4 increased the death rate of C2C12 cells, whereas KLF4 deficiency decreased the injury of C2C12 cells from heat stress conditions, suggesting that KLF4 might play an important role in cell injury induced by heat stress, KLF4 might be an immediate early response gene and could play an important role in cell injury induced by heat stress.