Analysis of Pax7 Expressing Myogenic Cells in Zebrafish Muscle Development, Injury, and Models of Disease
Analysis of Pax7 Expressing Myogenic Cells in Zebrafish Muscle Development, Injury, and Models of Disease
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DOI：
10.1002/dvdy.22745
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发表时间：
2011-11-01
影响因子：
2.5
通讯作者：
Ingham, Philip W.
中科院分区：
文献类型：
--
作者：
Seger, Claudia;Hargrave, Murray;Ingham, Philip W.
转录因子Pax 7是肌肉祖细胞和卫星细胞的标志物和调节剂，其有助于脊椎动物骨骼肌的胚胎发育和胚后生长，以及其修复和再生。在这里，我们确定Pax 7（+ve）的肌细胞在斑马鱼和表征其行为在胚后阶段。在受精后72小时（hpf），单核Pax 7（+ve）细胞可以首先被发现与肌纤维相关联。为了跟踪肌肉祖细胞在体内的行为，我们产生了在pax 7a或pax 3a启动子控制下表达荧光蛋白的转基因系。我们建立了一个损伤模型，使用心脏毒素注射和监测细胞增殖和生肌前体细胞和肌纤维中的生肌调节因子的表达损伤后，使用增殖标记物和转基因株。我们还分析了具有营养不良表型的动物中的Pax 7（+ve）细胞，并发现与野生型相比数量增加。Developmental Dynamics 240：2440-2451，2011. (C)2011 Wiley Periodicals，Inc.
The transcription factor Pax7 is a marker and regulator of muscle progenitors and satellite cells that contribute to the embryonic development and postembryonic growth of skeletal muscle in vertebrates, as well as to its repair and regeneration. Here, we identify Pax7(+ve) myogenic cells in the zebrafish and characterize their behavior in postembryonic stages. Mononucleate Pax7(+ve) cells can first be found associated with myofibers at 72 hours post fertilization (hpf). To follow the behavior of muscle progenitor cells in vivo, we generated transgenic lines expressing fluorescent proteins under the control of the pax7a or pax3a promoters. We established an injury model using cardiotoxin injection and monitored cell proliferation and myogenic regulatory factor expression in myogenic precursors cells and muscle fibers after injury using proliferation markers and the transgenic lines. We also analyzed Pax7(+ve) cells in animals with dystrophic phenotypes and found an increased number compared with wild-type. Developmental Dynamics 240: 2440-2451, 2011. (C) 2011 Wiley Periodicals, Inc.