Placental composition and surface area but not vascularization are altered by maternal protein restriction in the rat
Placental composition and surface area but not vascularization are altered by maternal protein restriction in the rat
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DOI：
10.1053/plac.2002.0858
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发表时间：
2003-01-01
期刊：
影响因子：
3.8
通讯作者：
Burton, GJ
中科院分区：
文献类型：
--
作者：
Doherty, CB;Lewis, RM;Burton, GJ
氨基酸是胎儿生长发育所必需的营养物质，尽管胎盘增大，母体蛋白质的限制会降低胎儿的生长。为了确定胎盘的结构是否发生了改变，从而损害了大鼠胎盘的功能，我们测定了母体蛋白限制对大鼠胎盘组成和血管化的影响。怀孕的大鼠随机接受正常蛋白质（20%）或等热量低（8%）蛋白质饮食。在妊娠第18天对胎盘进行研究。组织切片用生物素化的单纯斑叶（BS-1）凝集素染色，以便鉴定胎儿毛细血管，并使用体视学技术进行分析。蛋白质限制增加了胎盘总体积，但减少了迷宫组成的胎盘体积的比例。因此，迷路组织的总体积和胎、母血空间的总体积在两组间没有差异。蛋白质限制增加了母胎界面的表面积密度和总表面积，但没有改变胎儿毛细血管的表面积、直径和长度。这项研究发现，母体蛋白限制与胎盘大小的增加有关，而且这种增加伴随着一些（但不是全部）与功能相关的结构的增加。(C) 2002 Elsevier Science Ltd.版权所有。
Amino acids are essential nutrients for foetal growth and development, and maternal protein restriction decreases foetal growth despite increasing placental size. To determine if placental structure is altered so as to impair function despite increased size, the effect of maternal protein restriction on the composition and vascularization of the placenta in the rat was determined.Pregnant rats were randomized to receive either a normal protein (20 per cent) or an isocaloric low (8 per cent) protein diet. Placentae were studied at day 18 of gestation. Tissue sections were stained with biotinylated Bandeiraea simplicifolia (BS-1) lectin to allow identification of foetal capillaries, and analysed using stereological techniques.Protein restriction increased total placental volume, but decreased the proportion of the placental volume composed of labyrinth. Consequently the total volumes of labyrinthine tissue and of foetal and maternal blood space were not different between the two groups.Protein restriction increased the surface area density and total surface area of the materno-foetal interface, but did not alter foetal capillary surface area, diameter or length.This study found that maternal protein restriction is associated with an increase in placental size, and that this enlargement is accompanied by increases in some, but not all, structural correlates of function. (C) 2002 Elsevier Science Ltd. All rights reserved.