Vanadium pentoxide-coated ultrafine titanium dioxide particles induce cellular damage and micronucleus formation in V79 cells
Vanadium pentoxide-coated ultrafine titanium dioxide particles induce cellular damage and micronucleus formation in V79 cells
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DOI：
10.1080/15287390801989218
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Dopp, E.
中科院分区：
文献类型：
--
作者：
Bhattacharya, K.;Cramer, H.;Dopp, E.
表面处理的二氧化钛（TiO2）颗粒被五氧化二钒（V2O5）包裹，在工业上用于选择性催化反应，如从燃烧发电厂的废气中去除一氧化二氮（SCR工艺），以及用于增加植入物强度的生物材料。在本研究中，对未经处理的超细TiO2颗粒（锐钛矿，直径：30-50 nm）和五氧化二钒（V2O5）处理的锐钛矿颗粒在V79细胞（仓鼠肺成纤维细胞）中的细胞毒性和基因毒性进行了测试。采用台盼蓝排斥法评价颗粒的细胞毒性作用，微核（MN）法研究颗粒的遗传毒性作用。此外，采用电子自旋共振法（ESR）和细胞法测定硫代巴比妥酸反应物质（TBARS）测定活性氧（ROS）的生成。我们的研究结果表明，v2o5处理的TiO2颗粒比未处理的颗粒诱导更强的细胞和基因毒性作用。此外，与未处理的锐钛矿相比，v2o5锐钛矿的脱细胞和细胞自由基形成更为明显。因此，数据表明，v2o5处理的TiO2颗粒比天然锐钛矿更具活性，能够通过产生自由基诱导哺乳动物细胞DNA损伤。
Surface-treated titanium dioxide (TiO2) particles coated with vanadium pentoxide (V2O5) are used industrially for selective catalytic reactions such as the removal of nitrous oxide from exhaust gases of combustion power plants (SCR process) and in biomaterials for increasing the strength of implants. In the present study, untreated ultrafine TiO2 particles ( anatase, diameter: 30-50 nm) and vanadium pentoxide (V2O5)- treated anatase particles were tested for their cyto- and genotoxic effects in V79 cells ( hamster lung fibroblasts). Cytotoxic effects of the particles were assessed by trypan blue exclusion, while genotoxic effects were investigated by micronucleus (MN) assay. In addition, the generation of reactive oxygen species (ROS) was determined by the acellular method of electron spin resonance technique (ESR) and by the cellular technique of determination of thiobarbituric acid-reactive substances (TBARS). Our results demonstrate that V2O5-treated TiO2 particles induce more potent cyto- and genotoxic effects than untreated particles. Further, acellular and cellular radical formation was more pronounced with V2O5-anatase than untreated anatase. Thus, data indicate that V2O5-treated TiO2 particles were more reactive than natural anatase and capable of inducing DNA damage in mammalian cells through production of free radicals.