Oral feeding of Lactobacillus bulgaricus N45.10 inhibits the lung inflammation and airway remodeling in murine allergic asthma: Relevance to the Th1/Th2 cytokines and STAT6/T-bet
Oral feeding of Lactobacillus bulgaricus N45.10 inhibits the lung inflammation and airway remodeling in murine allergic asthma: Relevance to the Th1/Th2 cytokines and STAT6/T-bet
复制标题
DOI：
10.1016/j.cellimm.2019.103928
复制
发表时间：
2019-07-01
影响因子：
4.3
通讯作者：
Aimbire, F.
中科院分区：
文献类型：
--
作者：
Anatriello, E.;Cunha, M.;Aimbire, F.
哮喘是一种影响公众健康的慢性疾病。它影响气道，引起由Th 2型CD 4 T细胞介导的肺部炎症、嗜酸性粒细胞增多、粘液分泌过多和IgE升高。细胞因子和转录因子的失衡是哮喘的重要特征。益生菌作为一种治疗慢性病的药物越来越受到重视。因此，我们研究了保加利亚乳杆菌（Lb）在小鼠过敏性哮喘中的作用。BALB/c小鼠在第0天和第7天对卵清蛋白（OA）致敏，并从第14-28天用OA攻击。小鼠在致敏前7天接受Lb，并保持至第28天。Lb可降低肺组织中嗜酸性粒细胞浸润、粘液和胶原分泌、IgE产生、促炎细胞因子、TLR 4表达、GATA 3、STAT 6和ROR γ t。另外，Lb还可增加抗炎细胞因子T-bet和foxp 3的表达。Lb通过干预Th 1/Th 2细胞因子和STAT 6/T-bet转录因子，减轻过敏性哮喘气道炎症和气道重塑。
Asthma is a chronic disease with impacts on public health. It affects the airways causing pulmonary inflammation mediated by CD4 T cells type Th2, eosinophilia, mucus hypersecretion, and elevated IgE. The unbalance between cytokines and transcription factors is an important feature in asthma. Probiotics has gaining highlight as a therapy for chronic diseases. Thus, we investigate the Lactobacillus bulgaricus (Lb) effect in murine allergic asthma. BALB/c-mice were sensitized to ovalbumin (OA) on days 0 and 7 and were challenged from day 14-28 with OA. Mice received Lb seven days prior to sensitization and it was kept until day 28. The Lb attenuated the eosinophils infiltration, mucus and collagen secretion, IgE production, pro-inflammatory cytokines, TLR4 expression, GATA3, STAT6 and ROR gamma t in lung. Otherwise, Lb increased the anti-inflammatory cytokines, the T-bet and foxp3. Finally, Lb attenuated the allergic asthma-induced inflammation and airway remodeling by interfering on Th1/Th2 cytokines and STAT6/T-bet transcription factors.