Atrial natriuretic peptide modulates the hyperpolarization-activated current (If) in human atrial myocytes
Atrial natriuretic peptide modulates the hyperpolarization-activated current (If) in human atrial myocytes
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DOI：
10.1016/j.cardiores.2004.03.004
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发表时间：
2004-08-15
影响因子：
10.8
通讯作者：
Mugelli, A
中科院分区：
文献类型：
--
作者：
Lonardo, G;Cerbai, E;Mugelli, A
目标：心房拉伸与细胞兴奋性变化之间的关系已有充分记录。一旦拉伸，人心房肌细胞 (HuAM) 就会释放心房钠尿肽 (hANP)。 hANP (NPR) 受体与鸟苷酸环化酶 (GC) 活性偶联，并存在于 HuAM 上，但 hANP 的电生理效应很大程度上未知。我们研究了 hANP 对 If、HuAM 中存在的超极化激活电流以及潜在的细胞内通路的影响。方法：从心耳中分离出 HuAM，并用于膜片钳记录。结果：hANP 在 0.1 nM 时引起中点激活电位 (DeltaV(h)) 向 6.9 +/- 1.0 mV 的负电位发生显着且浓度依赖性的转变； 1 nM 时为 13.0 +/- 2.6 mV，10 nM 时为 15.3 +/- 2.2 mV（p
Objectives: The relationship between atrial stretching and changes in cell excitability is well documented. Once stretched, human atrial myocytes (HuAM) release atrial natriuretic peptide (hANP). Receptors for hANP (NPR) are coupled to a guanylyl cyclase (GC) activity, and are present on HuAM, but the electrophysiological effects of hANP are largely unknown. We investigated the effect of hANP on If, the hyperpolarization-activated current present in HuAM, and the underlying intracellular pathway. Methods: HuAM were isolated from atrial appendages and utilized for patch-clamp recording. Results: hANP caused a significant and concentration dependent shift of the midpoint activation potential (DeltaV(h)) toward less negative potentials of 6.9 +/- 1.0 mV at 0.1 nM; 13.0 +/- 2.6 mV at 1 nM and 15.3 +/- 2.2 mV at 10 nM (p