Molecular epidemiology of bovine Babesia spp. and Theileria orientalis parasites in beef cattle from northern and northeastern Thailand
Molecular epidemiology of bovine Babesia spp. and Theileria orientalis parasites in beef cattle from northern and northeastern Thailand
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DOI：
10.1016/j.parint.2015.10.005
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发表时间：
2016-02-01
影响因子：
1.9
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Jirapattharasate, Charoonluk;Moumouni, Paul Franck Adjou;Xuan, Xuenan
肉牛生产代表了泰国最大的牛群。它们的生产力受到诸如巴贝斯虫病和蜱虫病等蜱传疾病的限制。在本研究中，我们采用聚合酶链反应（PCR）测定了双臀巴贝斯虫、牛巴贝斯虫和东方巴贝斯虫的流行情况。对检测巴贝斯虫的遗传标记进行测序，确定巴贝斯虫属的同一性、相似性和7′的遗传多样性。胶。此外，还对泰国北部和东北部肉牛中发生上述原生动物寄生虫的危险因素进行了评估。在6个省共采集了329份肉牛血液样本。结果表明，肉牛寄生虫以东方弓形虫为主（30.1%），其次为双头弓形虫（13.1%）和牛弓形虫（5.5%）。总体而言，78.7%的筛选牛至少感染了上述寄生虫中的一种。同时感染巴贝斯虫和东方田鼠的比例为30.1%。三种寄生虫同时感染的比例为3.0%，但以双双小蠊和东方小蠊合并感染的比例最高（15.1%）。测序结果显示，不同牛源疟原虫RAP1基因和牛B. SBP2基因均有保守性。系统发育分析表明，从泰国北部和东北部分离的牛寄生虫中分离到的东方绦虫MPSP基因可分为5型和7型。缺乏蜱虫控制规划是泰国北部和东北部肉牛发生巴贝斯虫和东方绦虫感染的普遍危险因素。因此，我们建议对农民进行适当的蜱虫控制策略培训，并进一步研究7年的潜在病媒。并阐明与巴贝斯虫共感染对泰国北部和东北部牛肉东方绦虫感染致病性的影响。2015爱思唯尔爱尔兰有限公司版权所有。
Beef cattle production represents the largest cattle population in Thailand. Their productivity is constrained by tick-borne diseases such as babesiosis and theileriosis. In this study, we determined the prevalence of Babesia bigemina, Babesia bovis and Theileria orientalis using polymerase chain reaction (PCR). The genetic markers that were used for detection of the above parasites were sequenced to determine identities and similarity for Babesia spp. and genetic diversity of 7'. orientalis. Furthermore the risk factors for the occurrence of the above protozoan parasites in beef cattle from northern and northeastern parts of Thailand were assessed. A total of 329 blood samples were collected from beef cattle in 6 provinces. The study revealed that T. orientalis was the most prevalent (30.1%) parasite in beef cattle followed by B. bigemina (13.1%) and B. bovis (5.5%). Overall, 78.7% of the cattle screened were infected with at least one of the above parasites. Co-infection with Babesia spp. and T. orientalis was 30.1%. B. bigemina and T. orientalis were the most prevalent (15.1%) co-infection although triple infection with the three parasites was observed in 3.0% of the samples. Sequencing analysis revealed that B. bigemina RAP1 gene and B. bovis SBP2 gene were conserved among the parasites from different cattle samples. Phylogenetic analysis showed that the T. orientalis MPSP gene from parasites isolated from cattle in north and northeast Thailand was classified into types 5 and 7 as reported previously. Lack of tick control program was the universal risk factor of the occurrence of Babesia spp. and T. orientalis infection in beef cattle in northern and northeastern Thailand. We therefore recommend training of farmers on appropriate tick control strategies and further research on potential vectors for 7'. orientalis and elucidate the effect of co-infection with Babesia spp. on the pathogenicity of T. orientalis infection on beef in northern and northeastern Thailand. (C) 2015 Elsevier Ireland Ltd. All rights reserved.