Regulatory pathways of CYC‐like genes in patterning floral zygomorphy exemplified in Chirita pumila
Regulatory pathways of CYC‐like genes in patterning floral zygomorphy exemplified in Chirita pumila
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以 Chirita pumila 为例的 CYC 样基因在花对称性模式中的调控途径
DOI：
10.1111/jse.12574
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发表时间：
2021
影响因子：
3.7
通讯作者：
Wang Yin-Zheng
中科院分区：
文献类型：
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作者：
Liu Jing;Wu Jie;Yang Xia;Wang Yin-Zheng
花的左右对称被认为是对特定传粉者的一种重要适应。属于植物特异性TCP转录因子家族的CYCLOIDEA（CYC）样基因是花左右对称的关键调节因子。它们的表达分化导致花的两侧对称形式多样。然而，其表达分化的调控机制仍然未知。本研究选用Chirita pumilaD.唐，苦苣苔科的左右对称成员，作为一个模型来解决这个问题。突变体分析表明，矮生柑橘CYC基因（CpCYC）在控制花的左右对称性方面起重要作用。对CpCYC内含子和5 '调节区的进一步功能研究表明，899 bp的启动子区是CpCYC背侧特异性表达的关键决定因素。在该区域，我们利用电泳迁移率变动分析和瞬时表达系统，鉴定了三种具有假定结合因子的顺式调节元件，即生长素反应元件、WRKY结合位点、LEAFY结合位点和一个未知结合因子的元件。我们对这两个LEAFY结合位点进行了功能分析，发现LEAFY可以直接激活和调节CpCYC在花的两侧对称模式中的背侧特异性表达。这项研究将揭示CYC样基因空间特异性表达的调控和进化途径，以促进左右对称花的发育。
Floral zygomorphy is thought of as an essential adaptation to specific pollinators. TheCYCLOIDEA(CYC)‐like genes belonging to the plant‐specific TCP transcription factor family are the key regulators of floral zygomorphy. Their expression differentiations bring about diverse forms of floral zygomorphy. However, the regulatory mechanism underlying their expression differentiation remains unknown. In this study, we selectedChirita pumilaD. Don, a zygomorphic member of Gesneriaceae, as a model to address this question. Mutant analysis shows that theCYCgene inC.pumila(CpCYC) plays an important role in controlling floral zygomorphy. Further functional investigation ofCpCYCintron and 5′ regulatory regions indicates that an 899 bp promoter region is a key determinant of the dorsal‐specific expression ofCpCYC. In this region, using electrophoretic mobility shift assay and transient expression system, we identified three kinds ofcis‐regulatory elements with putative binding factors, that is, auxin responsive element, WRKY binding site, LEAFY binding site, and an element with an unknown binding factor. We undertook functional analyses of the two putative LEAFY binding sites, and found that LEAFY could directly activate and regulateCpCYCfor its dorsal‐specific expression in patterning floral zygomorphy. This research would shed significant light on the regulatory and evolutionary pathways underlying the spatial‐specific expression ofCYC‐like genes for the development of zygomorphic flowers.