Ultrasonic detection and developmental changes in calcification of the placenta during normal pregnancy in mice
Ultrasonic detection and developmental changes in calcification of the placenta during normal pregnancy in mice
复制标题
DOI：
10.1016/j.placenta.2004.05.010
复制
发表时间：
2005-02-01
期刊：
影响因子：
3.8
通讯作者：
Adamson, SL
中科院分区：
文献类型：
--
作者：
Alkirav, C;Lu, Y;Adamson, SL
高分辨率超声成像的小鼠胎盘在发展过程中发现高回声灶位于附近的母-胎盘界面在妊娠早期，近期，在胎盘迷路（交换区的胎盘）。使用茜素红染色在组织切片中识别的回声灶和钙沉积显示出与妊娠相似的定位和变化。由于在脱钙溶液中孵育子宫消除了钙沉积物和回声灶，因此钙沉积物引起回声灶。透射电子显微镜、X射线显微分析和电子衍射表明沉积物为羟基磷灰石钙晶体。在妊娠7.5-9.5天，在母体蜕膜和胎儿外胎盘锥滋养层巨细胞之间的边界处，钙沉积广泛而密集。绒毛膜-尿囊胎盘形成后（与第10.5天相似），钙化沉积物看起来更大，更稀薄，但仍位于母体蜕膜和胎盘滋养层巨细胞之间的边界。直到>= 15.5天（第18.5天为足月），在小鼠胎盘的胎盘中未观察到钙化沉积物。我们的结论是，沉积物的钙羟基磷灰石晶体在小鼠胎盘是可检测的高分辨率超声成像。这些沉积物提供了母体-胎盘界面的超声可检测标记物，其在妊娠7.5至9.5天之间的绒毛膜-尿囊胎盘建立期间特别突出。
High resolution ultrasound imaging of the mouse placenta during development revealed highly echogenic foci localized near the materno-placental interface in early gestation and, near term, in the placental labyrinth (the exchange region of the placenta). Echogenic foci and calcium deposits identified in histological sections using Alizarin red staining showed similar localization and changes with gestation. Calcium deposits caused the echogenic foci because incubating uteri in a decalcifying solution eliminated both the deposits and echogenic foci. Transmission electron microscopy, X-ray microanalysis, and electron diffraction were used to show that deposits were calcium hydroxyapatite crystals. Calcium deposits were extensive and densely packed at days 7.5-9.5 of gestation at the border between the maternal decidua and the fetal trophoblast giant cells of ectoplacental cone. After the formation of the chorio-allantoic placenta (similar to day 10.5), calcification deposits appeared larger and more rarefied but were still localized at the border between the maternal decidua and the fetal trophoblast giant cells of the placenta. Calcification deposits were not observed in the labyrinthine region of the mouse placenta until >= day 15.5 (day 18.5 is full term). We conclude that deposits of calcium hydroxyapatite crystals in the mouse placenta are detectable by high resolution ultrasound imaging. These deposits provide an ultrasound detectable marker of the maternal-placental interface that is particularly prominent during the establishment of the chorio-allantoic placenta between days 7.5 and 9.5 of gestation.