Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family
Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family
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KpnI 1.8 kb 家族成员中断与线粒体 DNA 同源的人类核序列
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
K. Shimada
中科院分区：
文献类型：
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作者：
H. Nomiyama;T. Tsuzuki;S. Wakasugi;M. Fukuda;K. Shimada
我们报道了与线粒体DNA（mtDNA）同源的几个DNA序列存在于人类核基因组中（Tsuzuki等（1983）Gene 25，223-229）。详细的Southern印迹分析表明，这样的序列之一是中断的重复序列约1.8 kb长，插入是分散的重复DNA序列的Kpn I 1.8 kb家族的一个成员。核苷酸序列分析表明，Kpn I的1.8 kb的DNA两侧是不完全的15碱基对（bp）的线粒体DNA的直接重复序列。该Kpn I 1.8 kb DNA在其3 '端具有富含A的序列，并且与已发表的与人Kpn I家族之一同源的cDNA序列之一以及与非洲绿色猴Kpn I家族之一同源的cDNA序列Kpn I-LS 1具有相当大的同源性。这些结构特征表明，Kpn I 1.8 kb DNA是一个可移动的元件，并通过RNA介导的过程插入mtDNA样序列。
We reported that several DNA sequences homologous to mitochondrial DNA (mtDNA) are present in the human nuclear genome (Tsuzuki et al. (1983) Gene 25, 223-229). Detailed Southern blot analyses revealed that one of such sequences is interrupted by a repetitive sequence about 1.8 kb long, and that the insert is one member of the dispersed repeated DNA sequences of the KpnI 1.8 kb family. Nucleotide sequence analysis showed that the KpnI 1.8 kb DNA is flanked with imperfect 15-base pair (bp) direct repeats of mtDNA. This KpnI 1.8 kb DNA has an A-rich sequence at its 3'-end, and has a considerable homology with one of the published cDNA sequences homologous to one of the human KpnI families and also to one of the African green monkey KpnI families, KpnI-LS1. These structural features suggest that the KpnI 1.8 kb DNA is a movable element and is inserted within the mtDNA-like sequence by an RNA-mediated process.