HISTOCHEMICAL EVIDENCE OF PROTEOLYSIS IN BONE - INFLUENCE OF PARATHORMONE
HISTOCHEMICAL EVIDENCE OF PROTEOLYSIS IN BONE - INFLUENCE OF PARATHORMONE
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DOI：
10.1177/11.6.734
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发表时间：
1963-01-01
影响因子：
3.2
通讯作者：
MIGICOVSKY, BB
中科院分区：
文献类型：
--
作者：
BELANGER, LF;MIGICOVSKY, BB
在雏鸡和大鼠骨的大小骨细胞水平上显示了蛋白水解性。通过在照相乳剂衬底中消化明胶。用甲状旁腺提取物对动物进行预处理，可增强该反应。加入氟化钠或亚铁氰化钾的孵育介质可抑制该反应。用乙醇甲醛-冰醋酸固定也能抑制该酶的活性。目前的结果似乎进一步证实和解释了成熟骨细胞的吸收潜力。
Proteolytic activity has been revealed at the level of the large osteocytes of young chick and rat bone. by digestion of gelatin in a photographic emulsion substrate. The reaction was enhanced by pretreatment of the animals with parathyroid extract. The reaction was inhibited by addition to the incubating medium of sodium fluoride or potassium ferrocyanide. It was also inhibited by fixation in ethanolformaldehyde-acetic acid. The present results seem to confirm and explain further the resorptive potential of the mature osteocytes.