Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing
Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing
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DOI：
10.1007/s11248-012-9596-0
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发表时间：
2012-12-01
影响因子：
3
通讯作者：
Sayanova, Olga
中科院分区：
文献类型：
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作者：
Ruiz-Lopez, Noemi;Haslam, Richard P.;Sayanova, Olga
二十碳五烯酸等长链多不饱和脂肪酸的合成和积累已经在转基因植物的种子中得到证实。然而，所获得的水平相对较低，表明需要进一步研究和更好地定义内源性脂质合成与非天然转基因编码活性之间的相互作用。在这项研究中，我们系统地比较了二十碳五烯酸生物合成途径的三种不同转基因配置，使用脂质组学分析来确定代谢瓶颈。我们还利用遗传杂交在拟南芥中堆叠了多达10个转基因。这些研究表明了优化转基因植物中目标脂肪酸积累的几种潜在方法。我们的数据显示，外源的C20多不饱和脂肪酸出乎意料地进入了次要的磷脂物种，并且磷脂依赖性的δ 6-去饱和酶也有明显的负代谢调节。总的来说，这项研究证实了迭代方法在植物脂合成代谢工程中的好处。
The synthesis and accumulation of long chain polyunsaturated fatty acids such as eicosapentaenoic acid has previously been demonstrated in the seeds of transgenic plants. However, the obtained levels are relatively low, indicating the need for further studies and the better definition of the interplay between endogenous lipid synthesis and the non-native transgene-encoded activities. In this study we have systematically compared three different transgenic configurations of the biosynthetic pathway for eicosapentaenoic acid, using lipidomic profiling to identify metabolic bottlenecks. We have also used genetic crossing to stack up to ten transgenes in Arabidopsis. These studies indicate several potential approaches to optimize the accumulation of target fatty acids in transgenic plants. Our data show the unexpected channeling of heterologous C20 polyunsaturated fatty acids into minor phospholipid species, and also the apparent negative metabolic regulation of phospholipid-dependent Delta 6-desaturases. Collectively, this study confirms the benefits of iterative approaches to metabolic engineering of plant lipid synthesis.