Analysis of Fascicular Structure and Connective Tissue Sheaths in Sural Nerve during Aging
Analysis of Fascicular Structure and Connective Tissue Sheaths in Sural Nerve during Aging
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DOI：
10.2478/afmnai-2014-0013
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发表时间：
2014-06-01
影响因子：
0.2
通讯作者：
Antic, Vladimir
中科院分区：
文献类型：
--
作者：
Kundalic, Braca;Ugrenovic, Sladana;Antic, Vladimir
为探讨腓肠神经外膜、周围膜和内膜结缔组织鞘在增龄过程中的变化，对10例9-80岁的腓肠神经标本进行了研究。使用标准程序将标本包埋在石蜡中，然后制作5 μ m厚的神经干横截面，并使用Masson三色染色法染色。在对神经的束状结构和连接鞘进行形态学分析后，使用数字图像分析软件“ImageJ”进行形态测量分析。每一个调查的情况下进行了分析，总神经，神经外膜和束横截面积，神经束膜指数，有髓轴突的体积密度和神经内膜含量的平均值。为了检验平均值差异的统计学显著性，我们对小的独立样本使用Student’s T检验，神经束的数目为5-13，而大多数神经束少于10个。在老化过程中，纤维束结构，包括纤维束的数量和纤维束/纤维束面积比，没有表现出显着的变化。老年组神经束膜厚度/神经束直径比在统计学上显著增加（p
The aim of our study was to analyze the changes of connective tissue sheaths of epi-, peri-and endoneurium of sural nerve during aging.The study was conducted on sural nerve samples of 10 cases aged 9-80 years. The specimens were embedded in paraffin using standard procedures, after which 5-mu m-thick cross-sections of nerve trunks were made and stained using Masson's trichrome staining. After morphological analysis of fascicular structure and connective sheaths of the nerve, morphometric analysis was conducted using the software for digital image analysis "ImageJ". Each investigated case was analyzed for total neural, epineurial and fascicular cross-section area, mean values of perineurial index, volume density of myelinated axons and of endoneurial content. To test the difference in mean values for statistical significance we used the Student's T-test for small independent sample.The number of fascicles was 5-13, while the majority of the nerves had less than 10 fascicles. Fascicular structure, which included the number of fascicles and epifascicular/fascicular area ratio, did not show significant changes during aging. Perineurial thickness / fascicle size ratio statistically significantly increased in the older investigated group (p