Indirect immunofluorescence of inactive centromeres as indicator of centromeric function
Indirect immunofluorescence of inactive centromeres as indicator of centromeric function
复制标题
非活性着丝粒的间接免疫荧光作为着丝粒功能的指标
DOI：
10.1007/bf00292655
复制
发表时间：
1986
期刊：
影响因子：
5.3
通讯作者：
O. Zuffardi
中科院分区：
文献类型：
--
作者：
D. Peretti;P. Maraschio;S. Lambiase;F. Curto;O. Zuffardi
文献中的两个先前的单个病例报告显示，在一条（X;X）和一条（9;11）双着丝粒染色体的非活性着丝粒部位存在或不存在着丝粒抗原。我们研究了九个不同的双着丝粒染色体使用抗着丝粒抗体和免疫荧光技术。在4个常染色体双着丝粒染色体中，非活性着丝粒始终为阳性，而由2条X染色体组成的双着丝粒染色体则为阳性或阴性，其中1例（X;Y）双着丝粒染色体为阴性。结果表明，X染色体的复制模式可能参与抑制免疫荧光的网站上的非活性着丝粒和双着丝粒染色体的一个着丝粒可能会失去其功能，但保留其抗原特性。这表明并非所有这些抗原都在微管-着丝粒相互作用中起作用。
Two previous single case reports from the literature showed the presence or absence of centromeric antigens at the site of the inactive centromeres in one (X;X) and in one (9;11) dicentric chromosome. We studied nine different dicentric chromosomes using anticentromeric antibodies and immunofluorescence techniques. In the four autosomal dicentrics the inactive centromere was consistently positive while the dicentrics composed of two X chromosomes were either positive or negative; one case of (X;Y) dicentric was negative. The results indicate that the X chromosome mode of replication may be involved in the suppression of immunofluorescence at the site of the inactive centromere and that one centromere of the dicentric chromosome may lose its function but conserve some of its antigenic properties. This indicates that not all these antigens play a rôle in the microtubules-centromere interaction.