The absorption, distribution, excretion, and metabolism of a single oral dose of O-ethyl O-4-nitrophenyl phenylphosphonothioate in hens.
The absorption, distribution, excretion, and metabolism of a single oral dose of O-ethyl O-4-nitrophenyl phenylphosphonothioate in hens.
复制标题
母鸡单次口服剂量的 O-乙基 O-4-硝基苯基苯基硫代膦的吸收、分布、排泄和代谢。
DOI：
10.1016/0041-008x(83)90175-8
复制
发表时间：
1983
影响因子：
3.8
通讯作者：
Ashry,MA
中科院分区：
文献类型：
--
作者：
Abou-Donia,MB;Reichert,BL;Ashry,MA
在成年母鸡中研究了单次经口给予10 mg/kg（LD 50）均匀苯基标记的[14 C]EPN（O-乙基O-4-硝基苯基[14 C]苯基硫代膦酸酯）的处置和代谢。用硫酸阿托品保护鸟类免受急性毒性。在每个时间间隔（天）处死3只给药母鸡：0.5、2、4、8、12。放射性从胃肠道吸收并分布在所有组织中。大部分剂量经尿-粪排泄物排泄（74%）。在呼出的CO2中仅检测到痕量放射性（0.2%）。大多数排泄的放射性物质被鉴定为苯基膦酸（PPA）、O-乙基苯基膦酸（EPPA）和O-乙基苯基硫代膦酸（EPPTA）。组织中的放射性在12天内达到11.8%的峰值。肝脏中的放射性浓度最高，其次是胆汁、肾脏、脂肪组织和肌肉。EPN是在脑、脊髓、坐骨神经、肾和血浆中鉴定的主要化合物。肝脏中的大部分放射性被鉴别为EPPA，其次是EPPTA和PPA。动力学研究表明，EPN从组织中以指数形式消失。EPN从血浆中消除的半衰期为16.5天，相当于0.04天-1的恒定速率值。EPN相对于血浆的相对驻留（RR）在肝脏中最短，在脂肪组织中最长，其次是坐骨神经和脊髓。
The disposition and metabolism of a single oral 10 mg/kg (LD50) of uniformly phenyl-labeled [14C]EPN (O-ethyl O-4-nitrophenyl [14C]phenylphosphonothioate) were studied in adult hens. The birds were protected from acute toxicity with atropine sulfate. Three treated hens were killed at each time interval (days): 0.5, 2, 4, 8, 12. Radioactivity was adsorbed from the gastrointestinal tract and distributed in all tissues. Most of the dose was excreted in the combined urinary-fecal excreta (74%). Only traces of the radioactivity (0.2%) were detected in expired CO2. Most of the excreted radioactive materials were identified as phenylphosphonic acid (PPA), O-ethyl phenylphosphonic acid (EPPA), and O-ethyl phenylphosphonothioc acid (EPPTA). Radioactivity in tissues reached a peak of 11.8% in 12 days. The highest concentration of radioactivity was present in the liver followed by bile, kidney, adipose tissue, and muscle. EPN was the major compound identified in brain, spinal cord, sciatic nerve, kidney, and plasma. Most of the radioactivity in the liver was identified as EPPA followed by EPPTA and PPA. Kinetic studies showed that EPN disappeared exponentially from tissues. The half-life of the elimination of EPN from plasma was 16.5 days corresponding to a constant rate value of 0.04 day−1. Relative residence (RR) of EPN relative to plasma was shortest in liver and longest in adipose tissue followed by sciatic nerve and spinal cord.