Bone response to mechanical loading in adult rats with collagen-induced arthritis
Bone response to mechanical loading in adult rats with collagen-induced arthritis
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DOI：
10.1016/j.bone.2004.05.002
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发表时间：
2004-10-01
期刊：
影响因子：
4.1
通讯作者：
Teshima, R
中科院分区：
文献类型：
--
作者：
Kameyama, Y;Hagino, H;Teshima, R
为了阐明炎症对骨对机械应力的反应的影响，我们使用胶原诱导的关节炎（CIA）大鼠模型进行了实验。实验中使用6月龄雌性Wistar大鼠。在所有CIA组中进行牛11型胶原致敏和1周后的额外致敏。使用四点弯曲装置施加载荷。在CIA和对照（CONT）组中，右侧胫骨在35 N（低组）、40 N（中组）或47 N（高组）下以2 Hz每周三次持续3周加载36个周期。从所有大鼠胫骨的骨膜和骨内膜表面收集组织形态计量学数据。胫骨骨膜表面分为外侧面和内侧面。计算骨形成表面（FS）、骨形成率（BFR）、骨沉积率（MAR）。在骨膜面的外侧面，加载与非加载胫骨的三个参数均显示出显著差异。CIA组的所有这些参数均显著低于CONT组，并且在施加的载荷和CIA之间观察到相互作用。在CONT组中，峰值应变与FS的左右差之间存在显著相关性。在骨膜面内侧，CIA组和CONT组加载侧FS、MAR和BFR均随力的增加而增加，但CONT组MAR增加。所有三个参数均显示负载和非负载时间段之间存在显著差异。在皮质内表面，在CONT组中，仅在负荷侧观察到与力相关的FS增加，并且负荷侧的FS显著高于非负荷侧。中情局大幅降低了所有三个参数。我们通过骨组织形态计量学研究了未治疗的CONT大鼠和CIA大鼠胫骨对机械负荷的反应，发现关节炎抑制了机械负荷诱导的骨形成。(C)2004年爱思唯尔公司All rights reserved.
To elucidate the effects of inflammation on the response of bone to mechanical stress, we performed experiments using a rat with collagen-induced arthritis (CIA) model. Six-month-old female Wistar rats were used in the experiment. Bovine type 11 collagen sensitization and additional sensitization after I week were performed in all CIA groups. Loads were applied using a four-point bending device. The right tibia was loaded in both CIA and control (CONT) groups at 35 N (low groups), 40 N (medium groups), or 47 N (high groups) for 36 cycles at 2 Hz three times per week for 3 weeks. Histomorphometrical data were collected from the periosteal and endosteal surfaces of the tibia in all rats. The tibia periosteal surface was subdivided into lateral and medial surfaces. Formation surface (FS), mineral apposition rate (MAR) and bone formation rate (BFR) were calculated. At lateral surface of periosteal surface, all three parameters showed significant differences between the loaded and nonloaded tibiae. All these parameters were significantly lower in CIA groups than in CONT groups, and interaction was seen between applied loading and CIA. There was a significant correlation between peak strain and the right-left difference of FS in the CONT groups. At medial surface of periosteal surface, there were force-related increase in FS, MAR, and BFR on the loaded side in both CIA and CONT groups, except MAR in the CONT group. All three parameters showed significant differences between the loaded and nonloaded tibiae. At endocortical surface, force-related increase was observed only in FS on the loaded side in CONT groups, and FS was significantly higher on the loaded side than the nonloaded side. CIA lowered all three parameters significantly. We examined the response to mechanical loading on the tibia in untreated CONT rats and rats with CIA by bone histomorphometry, and found that arthritis suppressed bone formation induced by mechanical loading. (C) 2004 Elsevier Inc. All rights reserved.