PURIFICATION OF DNA FROM FORMALDEHYDE FIXED AND PARAFFIN EMBEDDED HUMAN-TISSUE
PURIFICATION OF DNA FROM FORMALDEHYDE FIXED AND PARAFFIN EMBEDDED HUMAN-TISSUE
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DOI：
10.1016/0006-291x(85)90390-0
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
VOGELSTEIN, B
中科院分区：
文献类型：
--
作者：
GOELZ, SE;HAMILTON, SR;VOGELSTEIN, B
从保存的人体组织中分离DNA的能力将提供许多实验机会。可以从制备用于常规组织病理学检查的组织中提取DNA（即，用甲醛固定并包埋在石蜡中）。虽然提取的DNA不是完整的，但它是双链的，可被限制性内切酶切割，并且适用于分子生物学中使用的各种标准技术。
The ability to isolate DNA from preserved human tissues would provide numerous experimental opportunities. DNA can be extracted from tissues prepared for routine histopathological examination (i.e., fixed with formaldehyde and embedded in paraffin). Although the extracted DNA is not intact, it is double stranded, cleavable wih restriction endonucleases, and suitable for a variety of standard techniques used in molecular biology.