[Detection of cell apoptosis by MTT assay].

[Detection of cell apoptosis by MTT assay].
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DOI：
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发表时间：
2002-03
期刊：
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
影响因子：
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通讯作者：
C. Feng;Wen-li Ma;Yan-bing Song;Qiu-ye Guo;Qing-hua Wu;Wenling Zheng
C. Feng;Wen-li Ma;Yan-bing Song;Qiu-ye Guo;Qing-hua Wu;Wenling Zheng
中科院分区：
其他
文献类型：
--
作者：
C. Feng;Wen-li Ma;Yan-bing Song;Qiu-ye Guo;Qing-hua Wu;Wenling Zheng

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目的探讨MTT法检测细胞凋亡的可行性。方法K562细胞在含10%小牛血清和4 μ mol/L三氧化二砷的RPMI 1640培养基中生长。用As2O3诱导培养细胞凋亡，光镜、DNA凝胶电泳、MTT染色检测细胞凋亡情况。结果MTT染色法能准确检测细胞凋亡，并能将凋亡细胞与正常细胞和死亡细胞区分开来。结论MTT法检测细胞凋亡简便、实用。
OBJECTIVE To study the feasibility of using MTT assay to detect cell apoptosis. METHODS K562 cells were grown in RPMI 1640 medium supplemented with 10% calf serum and 4 micromol/L arsenic trioxide. Apoptosis was induced in the cultured cells by As2O3, and the cells were detected with optical microscope, DNA gel electrophoresis and MTT staining respectively. RESULT MTT staining could also accurately detect cell apoptosis, by which the apoptotic cells were easily distinguished from normal cells and dead cells. CONCLUSION MTT staining is simple, convenient and practical for detecting cell apoptosis.