MESSENGER-RNA IS TRANSLATED WHEN ASSOCIATED WITH THE CYTOSKELETAL FRAMEWORK IN NORMAL AND VSV-INFECTED HELA-CELLS
MESSENGER-RNA IS TRANSLATED WHEN ASSOCIATED WITH THE CYTOSKELETAL FRAMEWORK IN NORMAL AND VSV-INFECTED HELA-CELLS
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DOI：
10.1016/0092-8674(81)90276-2
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
PENMAN, S
中科院分区：
文献类型：
--
作者：
CERVERA, M;DREYFUSS, G;PENMAN, S
当用非离子去污剂提取悬浮生长的HeLa [人宫颈癌]细胞制备细胞骨架时，所有的多聚核糖体都与骨架结合; 80%的tRNA、主要部分的单核糖体和75%的细胞蛋白质存在于可溶性部分中。多聚核糖体的mRNA与细胞骨架结合，即使在提取前多聚核糖体在体内解体后，分子仍保持附着。所有主动翻译的信息分子都附着在框架上，但约1/4的poly（A）+ mRNA不含框架。将信息绑定到骨架上可能是翻译所必须的。在被VSV感染后，所有病毒多核糖体但不是所有感染细胞的病毒信息都与细胞骨架框架相关。脉冲追踪标记显示VSV消息最初与框架相关联，然后分离并停止翻译。已知病毒糖蛋白（G）的mRNA仅在与内质网结合的核糖体上翻译，也被去污剂提取的结构保留。结合多聚核糖体的内质网蛋白质亚结构可能是细胞骨架的一个组成部分。
When the cytoskeletal framework is prepared from suspension-grown HeLa [human cervical carcinoma] cells by extraction with nonionic detergent, all polyribosomes are associated with the framework; 80% of tRNA, the major portion of monoribosomes and 75% of the cell proteins are found in the soluble fraction. The mRNA of polyribosomes is bound to the cytoskeleton and the molecules remain attached even after polyribosomes are disassembled in vivo prior to extraction. All actively translating message molecules are attached to the framework, but about 1/4 of the poly(A)+ mRNA is free of the framework. The binding of message to the skeleton may be obligatory for translation. Upon infection with VSV, all viral polyribosomes but not all viral messages of the infected cell are associated with the cytoskeletal framework. Pulse-chase labeling shows that VSV messages initially associate with the framework and then later detach and cease translation. The mRNA for the viral glycoprotein (G), known to translate only on ribosomes bound to endoplasmic reticulum, is also retained by the detergent-extracted structure. The protein substructure of the endoplasmic reticulum which binds polyribosomes may be a component of the cytoskeletal framework.