The evening complex coordinates environmental and endogenous signals in Arabidopsis.
The evening complex coordinates environmental and endogenous signals in Arabidopsis.
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DOI：
10.1038/nplants.2017.87
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发表时间：
2017-06-26
期刊：
影响因子：
18
通讯作者：
Wigge PA
中科院分区：
文献类型：
--
作者：
Ezer D;Jung JH;Lan H;Biswas S;Gregoire L;Box MS;Charoensawan V;Cortijo S;Lai X;Stöckle D;Zubieta C;Jaeger KE;Wigge PA
植物通过对光和温度等信号的响应来调整其生长和发育，从而最大限度地提高其适应性。生物钟为植物提供了一种机制来预测诸如日出之类的事件并调整它们的转录程序。然而，植物通过内源性途径协调环境信号的潜在机制尚不完全清楚。通过RNA-seq和ChIP-seq实验，我们发现生物钟的夜间复合体（EC）在直接协调光合作用、生物钟、植物激素信号、生长和对环境的反应等数百个关键调控因子的表达中起着重要作用。我们发现EC结合全基因组目标的能力取决于温度。此外，光敏色素B （phyB）在EC结合的多个位点的共同存在提供了一种整合环境信息的机制。因此，我们的研究结果表明，EC在拟南芥协调内源和环境信号中起着核心作用。
Plants maximise their fitness by adjusting their growth and development in response to signals such as light and temperature. The circadian clock provides a mechanism for plants to anticipate events such as sunrise and adjust their transcriptional programmes. However, the underlying mechanisms by which plants coordinate environmental signals with endogenous pathways are not fully understood. Using RNA-seq and ChIP-seq experiments, we show that the evening complex (EC) of the circadian clock plays a major role in directly coordinating the expression of hundreds of key regulators of photosynthesis, the circadian clock, phytohormone signalling, growth and response to the environment. We find that the ability of the EC to bind targets genome-wide depends on temperature. In addition, co-occurrence of phytochrome B (phyB) at multiple sites where the EC is bound provides a mechanism for integrating environmental information. Hence, our results show that the EC plays a central role in coordinating endogenous and environmental signals in Arabidopsis.