Chronic stress enhances ibotenic acid-induced damage selectively within the hippocampal CA3 region of male, but not female rats
Chronic stress enhances ibotenic acid-induced damage selectively within the hippocampal CA3 region of male, but not female rats
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DOI：
10.1016/j.neuroscience.2004.01.049
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Wise, LS
中科院分区：
文献类型：
--
作者：
Conrad, CD;Jackson, JL;Wise, LS
这项研究的目的是评估海马体承受慢性应激后代谢挑战的能力。将N-甲基-D-天冬氨酸受体兴奋毒素（鹅膏蕈氨酸，IBO）注入海马CA 3区后，一段时间的限制为6小时/天/21天。在束缚结束后，当CA 3树突收缩持续（3至4天）时，将大鼠用IBO（或载体）输注到海马的CA 3区中。与对照组和生理盐水输注侧相比，应激雄性大鼠在IBO输注后显示出显著更多的CA 3损伤。此外，在急性应激（6小时约束）后3至4天，在CA 3区，也没有观察到IBO恶化的损害，在慢性应激后的CA 1区。女性也进行了检查，慢性应激并没有加剧IBO在CA 3区的损害。总的来说，这些结果表明，慢性应激损害了海马体在慢性应激方案消退后几天承受代谢挑战的能力。女性CA 3树突状回缩周围的条件是否代表脆弱性尚不清楚，需要进一步调查。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The purpose of this investigation was to assess the ability of the hippocampus to withstand a metabolic challenge following chronic stress. An N-methyl-D-aspartate receptor excitotoxin (ibotenic acid, IBO) was infused into the CA3 region of the hippocampus following a period of restraint for 6 h/day/21 days. Following the end of restraint when CA3 dendritic retraction persists (3 to 4 days), rats were infused with IBO (or vehicle) into the CA3 region of the hippocampus. Stressed male rats showed significantly more CA3 damage after IBO infusion relative to controls and the saline-infused side. Moreover, IBO-exacerbation of damage in males was not observed in the CA3 region 3 to 4 days after acute stress (6 h restraint), nor in the CA1 region after chronic stress. Females were also examined and chronic stress did not exacerbate IBO damage in the CA3 region. Overall, these results demonstrate that chronic stress compromises the ability of the hippocampus to withstand a metabolic challenge days after the chronic stress regimen has subsided in male rats. Whether the conditions surrounding CA3 dendritic retraction in females represents vulnerability is less clear and warrants further investigation. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.