Detection Efficiency of Ag Nanoparticle Labels for a Heart Failure Marker Using Linear and Square-Wave Anodic Stripping Voltammetry.
Detection Efficiency of Ag Nanoparticle Labels for a Heart Failure Marker Using Linear and Square-Wave Anodic Stripping Voltammetry.
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DOI：
10.3390/bios12040203
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发表时间：
2022-03-29
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影响因子：
--
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比较了线性扫描阳极溶出伏安法（LASV）和方波阳极溶出伏安法（SWASV）在纳米金属免疫分析中的应用。检查了两种单独的免疫测定：基于抗体之间相互作用的模型测定和心力衰竭标志物NT-proBNP的夹心测定。在这两种情况下，一种抗体与磁性微珠连接，一种抗体与球形Ag纳米颗粒标记连接。电化学检测是在纸质装置上进行的。研究的三个分析品质因数为测量精密度、校准灵敏度和检测限（LOD）。对于NT-proBNP测定，结果表明，在优化SWASV的潜在输入的脉冲幅度和频率后，检测效率显著高于LASV。具体而言，校准灵敏度提高了约40倍，平均变异系数降低了约40%，（LOD）降低至300.0 pM。最后，对于模型免疫测定，与LASV相比，观察到SWASV的LOD降低约10倍。
In this article, we compare linear sweep anodic stripping voltammetry (LASV) and square-wave anodic stripping voltammetry (SWASV) for detection of a nano metalloimmunoassay. Two separate immunoassays were examined: a model assay, based on interactions between antibodies, and a sandwich assay for the heart failure marker NT-proBNP. In both cases, one antibody is linked to a magnetic microbead, and one is linked to a spherical Ag nanoparticle label. Electrochemical detection is carried out on a paper device. The three analytical figures of merit studied were the precision of the measurements, the calibration sensitivity, and the limit of detection (LOD). For the NT-proBNP assay, the results show that after optimization of the pulse amplitude and frequency of the potential input for SWASV, the detection efficiency is substantially higher compared to LASV. Specifically, the calibration sensitivity increased by up to ~40 fold, the average coefficient of variation decreased by ~40%, and the (LOD) decreased to 300.0 pM. Finally, for a model immunoassay, a ~10-fold decrease in the LOD was observed for SWASV compared to LASV.
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影响因子：
8.9
作者：
Peng Y;Rabin C;Walgama CT;Pollok NE;Smith L;Richards I;Crooks RM
通讯作者：
Crooks RM
影响因子：
3.1
作者：
Kumar S;Vicente-Beckett V
通讯作者：
Vicente-Beckett V
影响因子：
8.9
作者：
Cunningham, Josephine C.;Kogan, Molly R.;Crooks, Richard M.
通讯作者：
Crooks, Richard M.
DOI：
10.1109/jtehm.2017.2740920
发表时间：
2017
影响因子：
3.4
作者：
Degregory PR;Tapia J;Wong T;Villa J;Richards I;Crooks RM
通讯作者：
Crooks RM
影响因子：
20.1
作者：
Roger VL
通讯作者：
Roger VL