Cartilage oligomeric matrix protein deficiency promotes early onset and the chronic development of collagen-induced arthritis.
Cartilage oligomeric matrix protein deficiency promotes early onset and the chronic development of collagen-induced arthritis.
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软骨寡聚基质蛋白缺乏症会促进早期发作和胶原蛋白诱导的关节炎的慢性发育。
DOI：
10.1186/ar2551
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发表时间：
2008
影响因子：
4.9
通讯作者：
Mattsson R
中科院分区：
文献类型：
--
作者：
Geng H;Carlsen S;Nandakumar KS;Holmdahl R;Aspberg A;Oldberg A;Mattsson R
软骨寡聚基质蛋白（COMP）是软骨中的一种五聚体蛋白。关节炎的发展，如胶原诱导性关节炎（CIA），涉及软骨作为靶组织。我们研究了CIA在COMP缺陷小鼠中的发展。将129/Sv背景中的COMP缺陷型小鼠与对慢性CIA易感的B10.Q小鼠回交10代。在胶原蛋白和胶原蛋白抗体诱导的关节炎模型中，测试COMP缺陷型和野生型小鼠的关节炎发作、发病率和严重程度。用酶联免疫吸附试验测定关节炎小鼠和野生型小鼠血清中抗II型胶原和抗COMP抗体以及血清COMP水平。COMP缺陷型小鼠表现出显着的早发性和增加CIA的严重程度在慢性期，而胶原蛋白II-抗体滴度在COMP缺陷型和野生型对照相似。在野生型小鼠中未发现COMP抗体。最后，COMP缺陷型和野生型小鼠对胶原抗体诱导的关节炎的反应相似，表明在关节炎的初始阶段，胶原II抗体如何与COMP缺陷型软骨相互作用没有差异。COMP缺乏促进慢性关节炎的早期发作和发展，但不影响胶原II自身免疫。这些发现强调了COMP在软骨稳定性中的重要性。
Cartilage oligomeric matrix protein (COMP) is a homopentameric protein in cartilage. The development of arthritis, like collagen-induced arthritis (CIA), involves cartilage as a target tissue. We have investigated the development of CIA in COMP-deficient mice. COMP-deficient mice in the 129/Sv background were backcrossed for 10 generations against B10.Q mice, which are susceptible to chronic CIA. COMP-deficient and wild-type mice were tested for onset, incidence, and severity of arthritis in both the collagen and collagen antibody-induced arthritis models. Serum anti-collagen II and anti-COMP antibodies as well as serum COMP levels in arthritic and wild-type mice were measured by enzyme-linked immunosorbent assay. COMP-deficient mice showed a significant early onset and increase in the severity of CIA in the chronic phase, whereas collagen II-antibody titers were similar in COMP-deficient and wild-type controls. COMP antibodies were not found in wild-type mice. Finally, COMP-deficient and wild-type mice responded similarly to collagen antibody-induced arthritis, indicating no difference in how collagen II antibodies interact with COMP-deficient cartilage during the initial stages of arthritis. COMP deficiency enhances the early onset and development of chronic arthritis but does not affect collagen II autoimmunity. These findings accentuate the importance of COMP in cartilage stability.
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影响因子：
4.9
作者：
Carlsen S;Nandakumar KS;Holmdahl R
通讯作者：
Holmdahl R
影响因子：
--
作者：
Nandakumar, Kutty Selva;Johansson, Bjorn P.;Holmdahl, Rikard
通讯作者：
Holmdahl, Rikard
影响因子：
2.2
作者：
Nandakumar, KS;Holmdahl, R
通讯作者：
Holmdahl, R
影响因子：
4.9
作者：
Liljander, Maria;Sallstrom, Mary-Ann;Mattsson, Ragnar
通讯作者：
Mattsson, Ragnar
影响因子：
4.8
作者：
Rosenberg, K;Olsson, H;Heinegård, D
通讯作者：
Heinegård, D