Methodology description for detection of cellular uptake of PVA coated superparamagnetic iron oxide nanoparticles (SPION) in synovial cells of sheep

Methodology description for detection of cellular uptake of PVA coated superparamagnetic iron oxide nanoparticles (SPION) in synovial cells of sheep
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DOI:
10.1016/j.jmmm.2005.02.076
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发表时间:
2005-05-01
影响因子:
2.7
通讯作者:
von Rechenberg, B
von Rechenberg, B
中科院分区:
材料科学3区
文献类型:
--
作者:
Schöpf, B;Neuberger, T;von Rechenberg, B

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报道了滑膜细胞中超顺磁性氧化铁纳米颗粒(SPION)的检测。在磁体的影响下(12小时),将滑膜细胞与1.5 mg/ml PVA包被的SPION孵育2、12、24和48小时。滑膜细胞对颗粒耐受良好,在12和24 h之间使用Turnbulls和普鲁士蓝反应容易检测到颗粒。(c)2005 Elsevier B. V.保留所有权利。
The detection of superparamagnetic iron oxide nanoparticles (SPION) in synoviocytes is reported. Synoviocytes were incubated for 2, 12, 24 and 48 h with 1.5 mg/ml of PVA coated SPION under the influence of magnets (12 h). Particles were well tolerated by the synoviocytes, were easily detected using the Turnbulls and Prussian blue reactions between 12 and 24 h. (c) 2005 Elsevier B.V. All rights reserved.