Rapid Detection of Escherichia Coliform Using A Series Electrode Piezoelectric Crystal Sensor
Rapid Detection of Escherichia Coliform Using A Series Electrode Piezoelectric Crystal Sensor
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DOI：
10.1080/00032719408000261
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发表时间：
1994-02
影响因子：
2
通讯作者：
Feng-jiang He;Wen-Hong Zhu;Q. Geng;L. Nie;S. Yao
中科院分区：
文献类型：
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作者：
Feng-jiang He;Wen-Hong Zhu;Q. Geng;L. Nie;S. Yao
摘要采用串联电极压电晶体传感器测定大肠杆菌。coli）。该方法简单、快速，只需一次样品稀释。E.杆菌通过该方法可以估计在10 - 106个细胞/ml的范围内。该过程是频率检测时间（FDT）方法。讨论了影响测定的因素。用该方法检测了30份纯培养物样品，结果在标准平板计数值的95%置信限内。频率检测时间与初始菌浓度的对数之间的相关系数为0.93。这与用于评估E的标准相对应。杆菌具有单次稀释和更快速结果的附加优点。
Abstract A series electrode piezoelectric crystal sensor was used to determine Escherichia coliform (E. coli.). This method is simple and rapid, and requires only a single sample dilution. E. coli. in the range of 10 - 106 cells per ml can be estimated by this method. The procedure is a frequency detection time (FDT) method. The factors which affect the determination are discussed. Thirty samples in pure culture were tested by this method and the results fell within the 95% confidence limits of the standard plate count values. A correlation coefficient of 0.93 was obtained between the frequency detection time and logarithm of initial bacterial concentration. This corresponds to the criteria used to evaluate E. coli. with the added advantage of single dilution and more rapid results.