Age-, sex-, and gonadal steroid-related changes in immunoreactive substance P in the rat anterior pituitary gland.
Age-, sex-, and gonadal steroid-related changes in immunoreactive substance P in the rat anterior pituitary gland.
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DOI：
10.1210/endo-117-4-1368
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发表时间：
1985-10
期刊：
影响因子：
4.8
通讯作者：
L. Depalatis;O. Khorram;S. Mccann
中科院分区：
文献类型：
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作者：
L. Depalatis;O. Khorram;S. Mccann
本文研究了大鼠垂体前叶(AP)免疫活性物质P(I-SP)浓度与年龄、性别、性腺激素和动情周期的关系。0.5月龄时，雄性和雌性AP-I-SP水平无差异。在2.0和5.0月龄时，AP I-SP浓度存在明显的性别差异，因为雄性AP的I-SP浓度分别是年龄匹配的雌性AP的3倍和8倍。成年大鼠长时间(6周)性腺切除后，与假手术对照组相比，雌性AP内I-SP含量升高，雄性AP内I-SP含量降低。用性腺类固醇填充的硅胶胶囊植入相同时间的去势动物，在雄性和雌性动物中产生了定性相同的反应；即与各自的对照组相比，去势动物的苯甲酸雌二醇水平降低，5-二氢睾酮丙酸酯水平升高。与各自的性腺切除控制值相比，丙酸睾丸酮治疗对AP I-SP水平没有影响。成年男性短期(8d)性腺切除对I-SP的AP浓度无影响。同样，与女性在阴道周期的发情第1天、第2天、发情前期或发情阶段的浓度相比，成年女性的性腺切除在改变AP I-SP浓度方面无效。这些数据表明，性腺类固醇在调节AP内I-SP浓度方面具有重要的生理学意义。我们假设I-SP是AP特有的，性腺类固醇直接作用于AP水平，影响多肽的合成和/或释放。
The interrelationship of anterior pituitary lobe (AP) immunoreactive substance P (I-SP) concentrations with age, sex, gonadal steroids, and estrous cyclicity in rats was examined. There was no difference between male and female AP I-SP levels at 0.5 month of age. At 2.0 and 5.0 months of age, a sex-linked difference in AP I-SP concentrations was evident, inasmuch as male APs contained approximately 3 and 8 times greater concentrations of I-SP, respectively, than APs from age-matched females. Long term (6 weeks) gonadectomy of adult rats resulted in an increase in I-SP concentrations in female APs and a decrease in the concentrations of the peptide in male APs compared to values in their respective sham-operated controls. Treatment of gonadectomized animals for the same length of time with gonadal steroid-filled Silastic capsule implants resulted in qualitatively identical responses in males and females; that is, estradiol benzoate decreased and 5 alpha-dihydrotestosterone propionate increased AP I-SP levels compared to the respective control values in castrates. Testosterone propionate treatment had no effect on AP I-SP levels compared with the respective gonadectomy control values. Short term (8 days) gonadectomy of adult males did not affect the AP concentration of I-SP. Likewise, gonadectomy of adult females was ineffective in altering the AP I-SP concentration compared with concentrations in females on diestrous day 1, diestrous day 2, proestrous, or estrous stages of the vaginal cycle. These data suggest that gonadal steroids are physiologically important in the regulation of I-SP concentrations in the AP. We hypothesize that I-SP is indigenous to the AP and that gonadal steroids act directly at the level of the AP to affect the synthesis and/or release of the peptide.