Genomic characterization, phylogenetic comparison and differential expression of the cyclic nucleotide-gated channels gene family in pear (Pyrus bretchneideri Rehd.)
Genomic characterization, phylogenetic comparison and differential expression of the cyclic nucleotide-gated channels gene family in pear (Pyrus bretchneideri Rehd.)
复制标题
梨（Pyrus bretchneideri Rehd.）环核苷酸门控通道基因家族的基因组特征、系统发育比较和差异表达
DOI：
10.1016/j.ygeno.2014.11.006
复制
发表时间：
2015-01-01
期刊：
影响因子：
4.4
通讯作者：
Wu, Juyou
中科院分区：
文献类型：
--
作者：
Chen, Jianqing;Yin, Hao;Wu, Juyou
环核苷酸门控通道(CNGC)家族参与多种阳离子的吸收，如钙离子，以调节植物的生长和响应生物和非生物胁迫。然而，在梨树等木本植物中，关于这个家族的信息要少得多。在此，我们对梨CNGC基因家族进行了全基因组鉴定和分析。系统发育分析表明，21个梨CNGC基因可分为I、II、III、IVA和IVB 5个类群。梨的大部分基因复制似乎是由节段复制引起的，发生在3294-3914万年前。进化分析表明，正向选择驱动了梨CNGC的进化。基序分析表明，第一组CNGC一般含有26个基序，是所有CNGC组中基序数量最多的。其中，每个组共有8个基序，表明这些结构域在CNGC活性中起着保守的作用。组织特异性表达分析表明，复制的CNGC基因的功能多样化是长期进化的主要特征。我们的结果还表明，在进化过程中，P-S6和PBC&铰链结构域是共同进化的。这些结果为我们进一步了解CNGC基因家族在高等植物中的功能、进化和表达分析提供了有价值的信息。(C)2014 Elsevier Inc.保留所有权利。
The cyclic nucleotide-gated channel (CNGC) family is involved in the uptake of various cations, such as Ca2+, to regulate plant growth and respond to biotic and abiotic stresses. However, there is far less information about this family in woody plants such as pear. Here, we provided a genome-wide identification and analysis of the CNGC gene family in pear. Phylogenetic analysis showed that the 21 pear CNGC genes could be divided into five groups (I, II, III, IVA and IVB). The majority of gene duplications in pear appeared to have been caused by segmental duplication and occurred 32.94-39.14 million years ago. Evolutionary analysis showed that positive selection had driven the evolution of pear CNGCs. Motif analyses showed that Group I CNGCs generally contained 26 motifs, which was the greatest number of motifs in all CNGC groups. Among these, eight motifs were shared by each group, suggesting that these domains play a conservative role in CNGC activity. Tissue-specific expression analysis indicated that functional diversification of the duplicated CNGC genes was a major feature of long-term evolution. Our results also suggested that the P-S6 and PBC & hinge domains had co-evolved during the evolution. These results provide valuable information to increase our understanding of the function, evolution and expression analyses of the CNGC gene family in higher plants. (C) 2014 Elsevier Inc. All rights reserved.