Bio-Templated Growth of Bone Minerals from Modified Simulated Body Fluid on Nanofibrous Decellularized Natural Tissues.
Bio-Templated Growth of Bone Minerals from Modified Simulated Body Fluid on Nanofibrous Decellularized Natural Tissues.
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DOI：
10.1166/jbn.2016.2202
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发表时间：
2016-04
影响因子：
2.9
通讯作者：
Mao C
中科院分区：
文献类型：
--
作者：
Yang M;Wang J;Zhu Y;Mao C
本研究中使用的小肠粘膜下层（SIS）膜是一种脱细胞的天然纳米纤维支架，来源于猪小肠的粘膜下层。它主要由I型胶原纤维组成。在此，我们研究了生物模板生长的羟基磷灰石（HAP）骨矿物质的SIS膜从一个修改的模拟体液（1.5 SBF）在体温下，即，在一个接近生理条件下，以评估其骨生物活性，膜在骨组织结合的能力，一旦植入体内。在1.5SBF中，HAP晶体在SIS膜上成功成核。晶体优先沿着构成SIS膜的胶原纤维成核。在整个培养过程中，HAP是主要的矿物晶相，而磷酸三钙（TCP）是次要的矿物晶相。我们还发现，矿化8小时最显着促进大鼠间充质干细胞（MSCs）的成骨分化，通过评估在MSCs的成骨标志物，包括碱性磷酸酶（早期标记）以及骨钙素和骨桥蛋白（晚期标记）的形成。因此，SIS膜具有良好的骨生物活性，一旦矿化，可以显著促进MSCs的成骨分化。
Small intestine submucosal (SIS) membrane used in this study is a decellularized, naturally occurring nanofibrous scaffold derived from a submucosal layer of porcine small intestine. It is predominantly composed of type I collagen fibers. Here we studied the bio-templated growth of hydroxylapatite (HAP) bone minerals on the SIS membrane from a modified simulated body fluid (1.5 SBF) at the body temperature, namely, under a near-physiological condition, in order to evaluate its bone bioactivity, the capability of the membrane in bonding with bone tissue once implanted in vivo. Minute HAP crystals were successfully nucleated on the SIS membranes from 1.5 SBF at the body temperature. The crystals were preferentially nucleated along the collagen fibers constituting the SIS membranes. HAP was the major crystalline mineral phase formed during the whole period of time and a minor crystalline phase of tricalcium phosphate (TCP) appeared after the membranes were incubated for 96 h. We also found that the mineralization for 8 h most significantly promoted the osteogenic differentiation of rat mesenchymal stem cells (MSCs) by evaluating the formation of osteogenic markers in MSCs including alkaline phosphatase (early stage marker) as well as osteocalcin and osteopontin (late stage markers). Hence, SIS membranes show excellent bone bioactivity and once mineralized, can significantly promote the osteogenic differentiation of MSCs.