Determination of iodide in seawater and urine by size exclusion chromatography with iodine-starch complex.
Determination of iodide in seawater and urine by size exclusion chromatography with iodine-starch complex.
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碘-淀粉复合物尺寸排阻色谱法测定海水和尿液中的碘化物。
DOI：
10.1016/s0021-9673(01)00776-2
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
X. Xu
中科院分区：
文献类型：
--
作者：
H. B. Li;F. Chen;X. Xu
建立了体积排阻色谱法测定碘离子的新方法。方法简便、灵敏度高、精密度好。碘转化为碘，然后用淀粉螯合，并使用Shim-pack DIOL-150（250×7.9 mm）尺寸排阻色谱柱（甲醇-0.01 mol l− 1磷酸水溶液（10：90，v/v）作为移动的流动相，流速为1.2 ml min− 1，UV检测波长为224 nm）从基质中分离。碘化物的校准曲线在1.0 ng ml− 1至100.0 ng ml− 1范围内呈线性，相关系数为0.9992（n=6）。检测限为0.2 ng ml−1。方法已成功地应用于海水和尿中碘的测定。方法的回收率为92%~ 103%，相对标准偏差为1.5%~ 3.7%。
A novel method for the determination of iodide by size exclusion chromatography was established. The method was simple and highly sensitive with good precision. Iodide was converted to iodine, then sequestered with starch, and separated from the matrix using a Shim-pack DIOL-150 (250×7.9 mm) size exclusion column with methanol–0.01 mol l−1aqueous phosphoric acid (10:90, v/v) as mobile phase at 1.2 ml min−1and UV detection at 224 nm. The calibration graph was linear from 1.0 ng ml−1to 100.0 ng ml−1for iodide with a correlation coefficient of 0.9992 (n=6). The detection limit was 0.2 ng ml−1. The method was successfully applied to the determination of iodide in seawater and urine. The recovery was from 92% to 103% and the relative standard deviation was in the range of 1.5% to 3.7%.