Rapid fabrication of detachable three-dimensional tissues by layering of cell sheets with heating centrifuge
Rapid fabrication of detachable three-dimensional tissues by layering of cell sheets with heating centrifuge
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DOI：
10.1002/btpr.2612
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发表时间：
2018-05-01
影响因子：
2.9
通讯作者：
Shimizu, Tatsuya
中科院分区：
文献类型：
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作者：
Haraguchi, Yuji;Kagawa, Yuki;Shimizu, Tatsuya
通过将温度降低到32 ℃以下，可以将温度响应性培养皿上的汇合培养细胞收获为完整的细胞片。三维（3-D）组织可以通过细胞片的分层来制造。在温度响应培养皿上得到的3-D多层细胞片-组织也可以通过仅降低温度而收获而没有任何损伤。为了缩短三维多层结构的制造时间，我们尝试将细胞片层在温度响应培养皿上离心。然而，当在22-23 ℃下用常规离心机将细胞片附着到培养表面时，细胞片由于其非细胞粘附而几乎不附着到表面。因此，在本研究中，我们开发了一种加热离心机。在36-37 ° C下离心（55 g）时，细胞片在5分钟内紧密粘附到培养皿上，没有显著的细胞损伤。此外，离心加速了细胞片层化过程。与没有离心的多层组织制造相比，加热离心缩短了五分之一的制造时间。此外，多层构建体最终通过降低温度从培养皿上分离。这种快速的组织制造方法将被用作组织工程和再生治疗领域的一种有价值的工具。(c)2018年美国化学工程师学会生物技术。程序：34：692-701，2018
Confluent cultured cells on a temperature-responsive culture dish can be harvested as an intact cell sheet by decreasing temperature below 32 degrees C. A three-dimensional (3-D) tissue can be fabricated by the layering of cell sheets. A resulting 3-D multilayered cell sheet-tissue on a temperature-responsive culture dish can be also harvested without any damage by only temperature decreasing. For shortening the fabrication time of the 3-D multilayered constructs, we attempted to layer cell sheets on a temperature-responsive culture dish with centrifugation. However, when a cell sheet was attached to the culture surface with a conventional centrifuge at 22-23 degrees C, the cell sheet hardly adhere to the surface due to its noncell adhesiveness. Therefore, in this study, we have developed a heating centrifuge. In centrifugation (55g) at 36-37 degrees C, the cell sheet adhered tightly within 5 min to the dish without significant cell damage. Additionally, centrifugation accelerated the cell sheet-layering process. The heating centrifugation shortened the fabrication time by one-fifth compared to a multilayer tissue fabrication without centrifugation. Furthermore, the multilayered constructs were finally detached from the dishes by decreasing temperature. This rapid tissue-fabrication method will be used as a valuable tool in the field of tissue engineering and regenerative therapy. (c) 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:692-701, 2018