Purinergic receptor expression and function in rat vagal sensory neurons innervating the stomach
Purinergic receptor expression and function in rat vagal sensory neurons innervating the stomach
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DOI：
10.1016/j.neulet.2019.05.017
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发表时间：
2019-07-27
影响因子：
2.5
通讯作者：
Holmes, Gregory M.
中科院分区：
文献类型：
--
作者：
Blanke, Emily N.;Stella, Salvatore L., Jr.;Holmes, Gregory M.
结状神经节（nodose ganglion，NG）是主要的副交感神经节，负责从内脏器官向中枢神经系统传递感觉信号，包括胃扩张或胃肠肽释放等消化信号。NG神经元对ATP和ADP的反应特征以及嘌呤能受体亚型的药理学询问以前已经进行了研究，但通常在未确定内脏来源的NG细胞中进行。在这项研究中，我们证实了P2X3和P2Y1受体的存在，并在胃神经支配NG神经元的P2X和P2Y反应的特点。应用ATP诱发的大内向电流和胞浆Ca2+增加胃神经NG神经元。尽管P2Y1受体的表达，ADP引起的电压门控钙通道只有轻微的调制。考虑到NG神经元对与疾病或神经损伤相关的共病的敏感性，在疾病或损伤状态下胃NG神经元的嘌呤能调制值得进一步研究。
The nodose ganglion (NG) is the main parasympathetic ganglion conveying sensory signals to the CNS from numerous visceral organs including digestive signals such as gastric distension or the release the gastrointestinal peptides. The response characteristics of NG neurons to ATP and ADP and pharmacological interrogation of purinergic receptor subtypes have been previously investigated but often in NG cells of undetermined visceral origin. In this study, we confirmed the presence of P2X3 and P2Y1 receptors and characterized P2X and P2Y responses in gastric-innervating NG neurons. Application of ATP-evoked large inward currents and cytosolic Ca2+ increases in gastric-innervating NG neurons. Despite the expression of P2Y1 receptors, ADP elicited only minor modulation of voltage-gated Ca2+ channels. Considering the sensitivity of NG neurons to comorbidities associated with disease or neural injury, purinergic modulation of gastric NG neurons in disease- or injury-states is worthy of further investigation.