Abundant expression and hemimethylation of C19MC in cell cultures from placenta-derived stromal cells
Abundant expression and hemimethylation of C19MC in cell cultures from placenta-derived stromal cells
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DOI：
10.1016/j.bbrc.2012.05.004
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发表时间：
2012-06-08
影响因子：
3.1
通讯作者：
Bullerdiek, Joern
中科院分区：
文献类型：
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作者：
Flor, Inga;Neumann, Armin;Bullerdiek, Joern
已知19号染色体microRNA簇（C19MC）的microRNA在胎盘中大量表达。他们的基因位于19号染色体的长臂上，似乎是一个大印迹区域的一部分。虽然目前已有的数据表明它们在胎盘中具有重要的功能，但在胎盘来源的间充质间质细胞（PDMSC）培养中，它们的一般表达模式尚无数据。令人惊讶的是，在妊娠早期和足月胎盘间充质间质细胞的组织培养中，qRT-PCR显示集群成员miR-517a-3p、miR-519a-3p和miR-520c-3p的丰富表达。因此，对甲基化模式的分析表明，这些细胞分别逃脱了父本等位基因的甲基化和表观遗传沉默。用去甲基化剂5-Aza-2'-脱氧胞苷处理绒毛膜绒毛基质细胞的结果证实了这一点。我们的结果提供了明确的证据，与之前的论文所提出的相反，C19MC成员在PDMSC中高度表达，这表明它们的胎盘特异性功能并不局限于滋养细胞。(c) 2012 Elsevier Inc.版权所有。
MicroRNAs of the chromosome 19 microRNA cluster (C19MC) are known to be abundantly expressed in the placenta. Their genes are located on the long arm of chromosome 19 and seem to be part of a large imprinted region. Although the data available so far suggest important functions in the placenta, no data are available on their general expression patterns in cultures of placenta-derived mesenchymal stromal cells (PDMSC). Surprisingly, qRT-PCR on tissue cultures from first-trimester and term placenta mesenchymal stromal cells showed an abundant expression of the cluster members miR-517a-3p, miR-519a-3p, and miR-520c-3p. Accordingly, analyses of methylation patterns suggested that these cells had escaped methylation and epigenetic silencing, respectively, of the paternal allele. This was confirmed by the results of treatment of chorionic villous stromal cells by the demethylating agent 5-Aza-2'-deoxycytidine. Our results offer clear evidence that, in contrast to what is suggested in previous papers, members of C19MC are highly expressed in PDMSC indicating that their placenta-specific functions are not restricted to the trophoblast. (c) 2012 Elsevier Inc. All rights reserved.