Collisionally induced fragmentation of protonated oligoalanines and oligoglycines.
Collisionally induced fragmentation of protonated oligoalanines and oligoglycines.
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质子化寡丙氨酸和寡甘氨酸的碰撞诱导断裂。
DOI：
10.1002/bms.1200200803
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Bursey,MM
中科院分区：
文献类型：
--
作者：
Yeh,RW;Grimley,JM;Bursey,MM
在混合仪器中，在最小的多次碰撞条件下，四丙氨酸和四甘氨酸的[M+H]+离子的碰撞引起的碎裂主要是它们的碎片，而不同于六、八丙氨酸和-甘氨酸的[M+H]+离子的碎裂；这些后者的碎裂是一种带状离子的分布，而且程度较小。这种差异可以基于对多肽中最碱性部位的碎裂进行控制来合理化，这可以通过考虑内部氢键来识别。当碰撞能量从10 eV增加到150 eV时，a出现了较低质量的带状离子；在一些情况下，由于较小的激子而产生的峰成为基峰。这些质子化多肽光谱中的离子分布可以作为一个基线，从这个基线可以探索构象对侧基重排和其他碎裂的影响。
In a hybrid instrument under minimal multiple‐collision conditions, the collision‐induced fragmentation of the [M + H]+ions of tetraalanine and tetraglycine are dominated by they2fragment, in distinction to the fragmentation of the [M + H]+ions of hexa‐ and octaalanine and ‐glycine; these latter fragmentations are instead a distribution ofbandyions, and to a lesser extentaions. This difference may be rationalized on the basis of control of the fragmentation by the most basic site in the peptide, which may be identified by taking internal hydrogen bonding into account. On increasing the collision energy from 10 to 150 eV,a, bandyions of lower mass appear; and in several cases a peak due to a smallerbion becomes the base peak. The ion distribution in the spectra of these protonated peptides serves as a baseline from which the effects of conformation on side‐group rearrangements and other fragmentations may be explored.