Functional analysis of a novel ABC transporter ABC4 from Magnaporthe grisea
Functional analysis of a novel ABC transporter ABC4 from Magnaporthe grisea
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DOI：
10.1111/j.1574-6968.2007.00937.x
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Chattoo, Bharat B.
中科院分区：
文献类型：
--
作者：
Gupta, Archna;Chattoo, Bharat B.
ATP结合盒（ABC）超家族的膜转运蛋白在多种植物病原真菌的致病过程中发挥重要作用。通过根癌农杆菌介导的遗传转化（ATMT）筛选稻瘟病菌致病性突变体，获得了一个新的ABC转运蛋白基因。ABC4基因全长5045 bp，编码1654个氨基酸。该突变体没有形成功能性附着胞，是非致病性的。当与野生型相比，突变体表现出增加某些抗真菌化合物和植物抗毒素的敏感性，这意味着ABC4的多药耐药（MDR），以及建立在主机中的作用。反转录PCR结果显示野生型菌株中有ABC4的表达，而突变株ABC4中没有表达。在实时PCR中，观察到ABC4的表达在各种测试药物的存在下增强。提示ABC4是M.这有助于真菌在感染期间科普细胞毒性环境。
The ATP-binding cassette (ABC) superfamily of membrane transporters has been implicated to play a role in pathogenesis in various phytopathogenic fungi. In an insertional mutagenesis screen for pathogenicity mutants of Magnaporthe grisea obtained via Agrobacterium tumefaciens-mediated transformation (ATMT), a novel gene belonging to the ABC transporter family was identified. The gene ABC4 was predicted to be 5045 bp in length coding for a protein of 1654 amino acids. The mutant did not form functional appressoria and was nonpathogenic. When compared with wild type, the mutant showed increased sensitivity to certain antifungal compounds and phytoalexins, implying the role of ABC4 in multidrug resistance (MDR) as well as establishment in the host. Reverse transcriptase PCR showed the expression of ABC4 in wild-type strain while it was absent in the mutant abc4. In real-time PCR, the expression of ABC4 was seen to be enhanced in the presence of various drugs tested. The data suggests that ABC4 is required for the pathogenicity of M. grisea, helping the fungus to cope with the cytotoxic environment during infection.