Cross-compatibility and the polyploidy of progenies in reciprocal backcrosses between diploid carnation (Dianthus caryophyllus L.) and its amphidiploid with Dianthus japonicus Thunb
Cross-compatibility and the polyploidy of progenies in reciprocal backcrosses between diploid carnation (Dianthus caryophyllus L.) and its amphidiploid with Dianthus japonicus Thunb
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DOI：
10.1016/j.scienta.2007.08.017
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发表时间：
2008-01-07
影响因子：
4.3
通讯作者：
Ohishi, Kazushi
中科院分区：
文献类型：
--
作者：
Nimura, Mikio;Kato, Juntaro;Ohishi, Kazushi
在康乃馨 (Dianthus caryophyllus L.) 的 6 个品系和来自 D. caryophyllus x Dianthus japonicus Thunb 的双二倍体品系之间进行了相互回交。以康乃馨为母本时，康乃馨的3个品系共获得24个后代。流式细胞术分析表明，它们由4个四倍体杂种植物、3个三倍体杂种植物和17个二倍体母本型子代组成。四倍体杂交植物仅从用作母本的康乃馨品系获得。以双二倍体为母本，用5个康乃馨品系授粉，获得137个后代。流式细胞仪分析证实它们均为三倍体杂种，PCR-RFLP分析也显示每个杂种均含有来自康乃馨和日本石竹的DNA片段。通过计算根尖细胞中的染色体数目来确认这些杂种的倍性水平。流式细胞术分析还显示，四倍体杂种具有三个康乃馨基因组和一个日本石竹基因组，表明康乃馨品系'02spROBA！产生未减少的雌配子。由于四倍体杂种和三倍体杂种均具有可育性，并且具有康乃馨的宽叶、茎强、耐热等一些有利的特性，因此有望用于康乃馨的未来育种。 (C) 2007 Elsevier B.V. 保留所有权利。
Reciprocal backcrosses were carried out between six lines of carnation (Dianthus caryophyllus L.) and one line of amphidiploid from D. caryophyllus x Dianthus japonicus Thunb. When carnation was used as the maternal parent, 24 progeny were obtained from three lines of carnation. Flow cytometric analysis revealed that they consisted of 4 tetraploid hybrid plants, 3 triploid hybrid plants and 17 diploid maternal-type progeny. The tetraploid hybrid plants were obtained from only one line of carnation used as maternal parent. When the amphidiploid was used as maternal parent, 137 progeny were obtained by pollination with 5 lines of carnation. All of them were confirmed to be triploid hybrids by flow cytometric analysis, PCR-RFLP analysis also revealed that each hybrid had DNA fragments originated from carnation and D. japonicus. The ploidy level of these hybrids was confirmed by counting chromosome numbers in root-tip cells. Flow cytometric analysis also revealed that the tetraploid hybrids had three genomes of carnation and one genome of D. japonicus, suggesting that the carnation line '02spROBA! produced unreduced female gametes. Since not only the tetraploid hybrids but also the triploid ones were fertile and have some of the profitable characters of D. japonicus such as broad leaf, strong stem and heat tolerance, they are expected to use for future breeding in carnation. (C) 2007 Elsevier B.V. All rights reserved.