Nonrandom chromosomal imbalances in human ovarian surface epithelial cells immortalized by HPV16-E6E7 viral oncogenes
Nonrandom chromosomal imbalances in human ovarian surface epithelial cells immortalized by HPV16-E6E7 viral oncogenes
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DOI：
10.1016/s0165-4608(01)00473-3
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发表时间：
2001-10-15
影响因子：
--
通讯作者：
Wong, YC
中科院分区：
文献类型：
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作者：
Tsao, SW;Wong, N;Wong, YC
我们之前使用 HPV16E6E7 ORF 永生化了人卵巢表面上皮 (HOSE) 细胞。为了鉴定细胞永生化过程中涉及的关键遗传事件，通过比较基因组杂交分析了五种 HOSE 细胞系永生化的基因组图谱。我们的结果表明，永生化后的 HOSE 细胞普遍存在染色体失衡。在永生HOSE细胞中发现的常见染色体失衡是：+19q13.1（5/5系）、-13q12与qter相似（4/5系）、+5q15与q33相似（3/5系）、+20q11.2与q13.2相似（3/5系）和-22q11.2与qter相似（315系）。在五个永生 HOSE 细胞系中的两个中检测到的其他染色体失衡包括与 q13 类似的 1 号染色体和 11q12 染色体增加，以及与 qter 类似的 2p、4q、8p、10p 和 11q14 染色体损失。永生化前在HOSE细胞中观察到的染色体失衡包括与p11.2相似的-8pter、与qter相似的-11q23、与qter相似的-13q12和+19，这可能代表细胞永生化过程中的早期遗传事件。在永生化各个阶段的一种 HOSE 细胞系 (HOSE 6-3) 中检查了基因组图谱。危机后 HOSE 6-3 细胞的基因组谱基本稳定。经过广泛的培养期后，在永生化 HOSE 细胞中检测到了一些额外的染色体失衡，包括与 q23 相似的 +11pter、与 qter 相似的 -15q23 和与 qter 相似的 +17q12。永生化 HOSE 细胞中非随机染色体失衡的鉴定可能有助于鉴定含有参与人卵巢表面上皮细胞永生化的基因的特定染色体。 (C) 2001 Elsevier Science Inc. 保留所有权利。
We had previously immortalized human ovarian surface epithelial (HOSE) cells using HPV16E6E7 ORFs. In order to identify crucial genetic events involved during cell immortalization, the genomic profile of immortalization of five HOSE cell lines was analyzed by comparative genomic hybridization. Our results showed that chromosomal imbalance was common in HOSE cells after immortalization. The common chromosomal imbalances identified in immortal HOSE cells are: +19q13.1 (5/5 lines), -13q12 similar to qter (4/5 lines), +5q15 similar to q33 (3/5 lines), +20q11.2 similar to q13.2 (3/5 lines) and -22q11.2 similar to qter (315 lines). Other chromosomal imbalances, which were detected in two of the five immortal HOSE cell lines, included gains on chromosome 1 and 11q12 similar to q13, and losses on 2p, 4q, 8p, 10p and 11q14 similar to qter. The chromosomal imbalances observed in HOSE cells before immortalization include -8pter similar to p11.2, -11q23 similar to qter, -13q12 similar to qter and +19 which may represent early genetic events during cell immortalization. The genomic profile was examined in one HOSE cell line (HOSE 6-3) at various stages of immortalization. The genomic profiles of HOSE 6-3 cells after crisis were largely stable. A few additional chromosomal imbalances were detected in the immortalized HOSE cells after an extensive culture period including +11pter similar to q23, -15q23 similar to qter, and +17q12 similar to qter. Identification of nonrandom chromosomal imbalance in immortalized HOSE cells may facilitate the identification of specific chromosomes harboring genes involved in the immortalization of human ovarian surface epithelial cells. (C) 2001 Elsevier Science Inc. All rights reserved.