Macaques with rapid disease progression and simian immunodeficiency virus encephalitis have a unique cytokine profile in peripheral lymphoid tissues
Macaques with rapid disease progression and simian immunodeficiency virus encephalitis have a unique cytokine profile in peripheral lymphoid tissues
复制标题
DOI：
10.1128/jvi.75.9.4448-4452.2001
复制
发表时间：
2001-05-01
影响因子：
5.4
通讯作者：
Lackner, AA
中科院分区：
文献类型：
--
作者：
Orandle, AS;Williams, KC;Lackner, AA
在猿猴免疫缺陷病毒（SIV）感染的艾滋病猕猴模型中研究了宿主细胞因子反应对病毒载量、疾病进展和神经损伤的影响。通过半定量逆转录 PCR 评估 5 只对照动物和 28 只感染 SIVmac251 动物的淋巴结中细胞因子基因表达（白介素 1 β [IL-1 β]、IL 2、IL-6、IL-10、γ 干扰素 [IFN-γ] 和肿瘤坏死因子 α [TNF-α]）和病毒载量。 艾滋病的末期。与对照组相比，受感染的动物显示出更高的 IFN-γ、IL-6 和 IL-10 mRNA 表达。疾病进展快速（存活 < 200 天）或缓慢/正常（存活 > 200 天）的动物之间所有细胞因子的水平相当。然而，在快速进展者中，八只患有 SN 脑炎的动物具有独特的细胞因子谱（IL-2、IL-6 和 IFN-γ 增加），与较高的病毒载量相关。这些观察结果证明宿主细胞因子反应可能影响 SIV 神经发病机制，而与疾病进展无关。
The influence of host cytokine response on viral load, disease progression, and neurologic lesions was investigated in the simian immunodeficiency virus (SIV)-infected macaque model of AIDS. Cytokine gene expression (interleukin-1 beta [IL-1 beta], IL 2, IL-6, IL-10, gamma interferon [IFN-gamma], and tumor necrosis factor alpha [TNF-alpha]) and viral Loads were evaluated by semiquantitative reverse transcription-PCR in lymph nodes of 5 control animals and 28 animals infected with SIVmac251 at the terminal stages of AIDS. Infected animals showed higher expression of IFN-gamma, IL-6, and IL-10 mRNAs compared with controls. Levels of all cytokines were comparable between animals with rapid (survival, < 200 days) or slow/normal (survival, > 200 days) disease progression. However, among rapid progressors, the eight animals with SN encephalitis had a unique cytokine profile (increased IL-2, IL-6, and IFN-gamma) that was associated with higher viral loads. These observations provide evidence that host cytokine responses may influence SIV neuropathogenesis independent of disease progression.