Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels
Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels
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DOI：
10.1038/s41598-019-52730-4
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发表时间：
2019-11-08
影响因子：
4.6
通讯作者：
Balkhy, Hanan H.
中科院分区：
文献类型：
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作者：
Alharbi, Naif Khalaf;Qasim, Ibrahim;Balkhy, Hanan H.
MERS冠状病毒血清阴性和血清阳性的骆驼接受单次肌肉注射ChAdOx1 MERS，这是一种表达MERS冠状病毒刺突蛋白的复制缺陷腺病毒载体疫苗，其他组接受对照疫苗接种。将自然感染MERSCoV活动性的传染性骆驼与接种疫苗的骆驼按1：2(感染：接种)的比例共养14天，监测流鼻液和病毒滴度。总体而言，接种疫苗减少了病毒脱落和鼻涕(分别为p=0.0059和p=0.0274)。疫苗增强了血清阳性骆驼的抗体反应；这些骆驼的平均年龄高于血清阴性的骆驼。年长的血清阴性骆驼对疫苗的反应比年轻的动物更强烈；在鼻拭子中检测到中和抗体。需要进一步的工作来优化年轻血清阴性骆驼的疫苗方案。
MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, a replication-deficient adenoviral vectored vaccine expressing MERS-CoV spike protein, with further groups receiving control vaccinations. Infectious camels with active naturally acquired MERSCoV infection, were co-housed with the vaccinated camels at a ratio of 1:2 (infected:vaccinated); nasal discharge and virus titres were monitored for 14 days. Overall, the vaccination reduced virus shedding and nasal discharge (p = 0.0059 and p = 0.0274, respectively). Antibody responses in seropositive camels were enhancedby the vaccine; these camels had a higher average age than seronegative. Older seronegative camels responded more strongly to vaccination than younger animals; and neutralising antibodies were detected in nasal swabs. Further work is required to optimise vaccine regimens for younger seronegative camels.