Fluorescently labeled cellulose nanocrystals for bioimaging applications
Fluorescently labeled cellulose nanocrystals for bioimaging applications
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DOI：
10.1021/ja076196l
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发表时间：
2007-11-14
影响因子：
15
通讯作者：
Roman, Maren
中科院分区：
文献类型：
--
作者：
Dong, Shuping;Roman, Maren
纤维素纳米晶在纳米医学领域具有广阔的应用前景。为了能够在体外和体内的研究中使用荧光技术，通过三步反应用荧光素-5‘-异硫氰酸酯(FITC)标记了纤维素纳米晶，包括纳米晶表面的环氧活化，用氢氧化铵开环，以及FITC分子与伯氨基的偶联。用UV/Vis光谱测定了标记的纤维素纳米晶的FITC含量。用所述方法得到的FITC含量为0.03 mmol/g纤维素，相当于每1000个无氢葡萄糖单位中有5个FITC含量。
Cellulose nanocrystals are promising candidates for applications in nanomedicine. To enable the use of fluorescence techniques in in vitro and in vivo studies, cellulose nanocrystals were labeled with fluorescein-5'-isothiocyanate (FITC) via a three-step reaction, involving epoxy activation of the nanocrystal surface, opening of the epoxy rings with ammonium hydroxide, and coupling of FITC molecules to the primary amino groups. The FITC content of the labeled cellulose nanocrystals was determined by UV/vis spectroscopy. A FITC content of 0.03 mmol/g of cellulose, equivalent to 5 FITC moieties per 1000 anhydroglucose units, was obtained using the described method.