Cyclic AMP regulates the rate of differentiation of oligodendrocytes without changing the lineage commitment of their progenitors.
Cyclic AMP regulates the rate of differentiation of oligodendrocytes without changing the lineage commitment of their progenitors.
复制标题
环 AMP 调节少突胶质细胞的分化率，而不改变其祖细胞的谱系承诺。
DOI：
10.1016/0012-1606(89)90047-x
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
McMorris,FA
中科院分区：
文献类型：
--
作者：
Raible,DW;McMorris,FA
少突胶质细胞在大鼠脑细胞的原代培养物中分化的特定时间表类似于体内的神经胶质细胞。我们表明，这种发展的步伐加快了环AMP类似物二丁酰cAMP（dbcAMP）或8-溴cAMP的加入。二丁酰环磷酸腺苷还抑制A2 B5阳性少突胶质细胞2型星形胶质细胞（O-2A）祖细胞的DNA合成，这与体内和体外观察到的增殖停止和分化开始之间的关系一致。用dbcAMP处理培养物对变成少突胶质细胞而不是2型星形胶质细胞的O-2A祖细胞的比例没有影响，因此不影响祖细胞谱系决定。因此，环AMP类似物加速细胞的分化，显然已经确定成为少突胶质细胞。
Oligodendrocytes differentiate in primary cultures of rat brain cells on a specific schedule similar to that observedin vivo. We show that the pace of this developmental schedule is accelerated by the addition of the cyclic AMP analogs dibutyryl cAMP (dbcAMP) or 8-bromo cAMP. Dibutyryl cAMP also inhibits DNA synthesis in A2B5-positive oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells, consistent with the relationship between cessation of proliferation and onset of differentiation observedin vivoandin vitro. Treatment of cultures with dbcAMP has no effect on the proportion of O-2A progenitors that become oligodendrocytes rather than type 2 astrocytes and thus does not affect progenitor lineage decisions. Thus, cyclic AMP analogs accelerate the differentiation of cells apparently already determined to become oligodendrocytes.